Rabbit monoclonal [EPR392] to DNA PKcs - BSA and Azide free
WB, ICC/IFmore details Unsuitable for:
Flow Cyt,IHC-P or IP
K562, Molt4, MCF7, SH-SY5Y, 293T and PC3 cell lysates.
ab174573 is a PBS-only buffer formulated version of ab133516, containing no BSA or sodium azide, ideal for antibody labeling. Please refer to ab133516 for information on protocols, dilutions, and image data.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Detects a band of approximately 460 kDa (predicted molecular weight: 469 kDa).
Use at an assay dependent concentration.
Is unsuitable for Flow Cyt,IHC-P or IP.
Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D.
Phosphorylated upon DNA damage, probably by ATM or ATR. Autophosphorylated on Thr-2609, Thr-2638 and Thr-2647. Thr-2609 is a DNA damage-inducible phosphorylation site (inducible with ionizing radiation, IR). Autophosphorylation induces a conformational change that leads to remodeling of the DNA-PK complex, requisite for efficient end processing and DNA repair. S-nitrosylated by GAPDH.
DNA dependent protein kinase catalytic subunit antibody
DNA PK catalytic subunit antibody
DNA-dependent protein kinase catalytic subunit antibody
DNA-PK catalytic subunit antibody
DNAPK catalytic subunit antibody
DNPK 1 antibody
Hyper radiosensitivity of murine scid mutation, complementing 1 antibody
Hyperradiosensitivity complementing 1, mouse, homolog of 1 antibody
HYRC 1 antibody
Protein Kinase DNA Activated Catalytic Polypeptide antibody
XRCC 7 antibody
Western blot - Anti-DNA PKcs antibody [EPR392] - BSA and Azide free (ab174573)
This WB data was generated using the same anti-DNA PKcs antibody clone, EPR392, in a different buffer formulation (cat# ab133516).
Lane 1: Wild type HAP1 whole cell lysate (40 µg) Lane 2: DNA PKcs knockout HAP1 whole cell lysate (40 µg) Lane 3: K562 whole cell lysate (20 µg) Lane 4: SHSY5Y whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab133516 observed at 460 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab133516 was shown to specifically react with DNA PKcs when DNA PKcs knockout samples were used. Wild-type and DNA PKcs knockout samples were subjected to SDS-PAGE. Ab133516 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Other - Anti-DNA PKcs antibody [EPR392] - BSA and Azide free (ab174573)
This kD data was generated using the same anti-DNA PKcs antibody clone, EPR392, in a different buffer formulation (cat# ab133516).
Equilibrium disassociation constant (KD) Learn more about KD