Anti-DNA PKcs (phospho S2056) antibody - ChIP Grade (ab18192)

Rabbit polyclonal DNA PKcs (phospho S2056) antibody. Validated in WB, ELISA, IHC, Flow Cyt, ChIP, ICC/IF and tested in Human. Cited in 97 publication(s). Independently reviewed in 13 review(s).

Publishing research using ab18192? Please let us know so that we can cite the reference in this datasheet.

ab18192 has been referenced in 100 publications.

  • Liu S  et al. Genotoxic stress causes the accumulation of DNA-dependent protein kinase catalytic subunit phosphorylated at serine 2056 at nuclear speckles and alters pre-mRNA alternative splicing. FEBS Open Bio 9:304-314 (2019). PubMed: 30761255
  • Munnur D  et al. NR4A Nuclear Receptors Target Poly-ADP-Ribosylated DNA-PKcs Protein to Promote DNA Repair. Cell Rep 26:2028-2036.e6 (2019). PubMed: 30784586
  • Roch B  et al. Cernunnos/Xlf Deficiency Results in Suboptimal V(D)J Recombination and Impaired Lymphoid Development in Mice. Front Immunol 10:443 (2019). PubMed: 30923523
  • Hafsi H  et al. Combined ATR and DNA-PK Inhibition Radiosensitizes Tumor Cells Independently of Their p53 Status. Front Oncol 8:245 (2018). PubMed: 30057890
  • Johnson TI  et al. Fumarate hydratase loss promotes mitotic entry in the presence of DNA damage after ionising radiation. Cell Death Dis 9:913 (2018). PubMed: 30190474
  • He H  et al. UHRF1 depletion sensitizes retinoblastoma cells to chemotherapeutic drugs via downregulation of XRCC4. Cell Death Dis 9:164 (2018). WB . PubMed: 29415984
  • Naumann M  et al. Impaired DNA damage response signaling by FUS-NLS mutations leads to neurodegeneration and FUS aggregate formation. Nat Commun 9:335 (2018). WB ; Human . PubMed: 29362359
  • Deraska PV  et al. NF-?B inhibition by dimethylaminoparthenolide radiosensitizes non-small-cell lung carcinoma by blocking DNA double-strand break repair. Cell Death Discov 4:10 (2018). PubMed: 29531807
  • Eccles LJ  et al. Inhibition of non-homologous end joining in Fanconi Anemia cells results in rescue of survival after interstrand crosslinks but sensitization to replication associated double-strand breaks. DNA Repair (Amst) 64:1-9 (2018). PubMed: 29459202
  • Li M  et al. APE1 deficiency promotes cellular senescence and premature aging features. Nucleic Acids Res 46:5664-5677 (2018). PubMed: 29750271
  • Morotomi-Yano K  et al. Dynamic behavior of DNA topoisomerase IIß in response to DNA double-strand breaks. Sci Rep 8:10344 (2018). PubMed: 29985428
  • Tsuji T  et al. CC-115, a dual inhibitor of mTOR kinase and DNA-PK, blocks DNA damage repair pathways and selectively inhibits ATM-deficient cell growthin vitro. Oncotarget 8:74688-74702 (2017). PubMed: 29088817
  • Saha J  et al. Examining DNA Double-Strand Break Repair in a Cell Cycle-Dependent Manner. Methods Enzymol 591:97-118 (2017). PubMed: 28645381
  • Dubois JC  et al. A phosphorylation-and-ubiquitylation circuitry driving ATR activation and homologous recombination. Nucleic Acids Res 45:8859-8872 (2017). PubMed: 28666352
  • Zhu S  et al. Protein phosphatase 1 and phosphatase 1 nuclear targeting subunit-dependent regulation of DNA-dependent protein kinase and non-homologous end joining. Nucleic Acids Res 45:10583-10594 (2017). PubMed: 28985363
  • Vormoor B  et al. Sensitizing Ewing sarcoma to chemo- and radiotherapy by inhibition of the DNA-repair enzymes DNA protein kinase (DNA-PK) and poly-ADP-ribose polymerase (PARP) 1/2. Oncotarget 8:113418-113430 (2017). PubMed: 29371919
  • Wald-Altman S  et al. A differential autophagy-dependent response to DNA double-strand breaks in bone marrow mesenchymal stem cells from sporadic ALS patients. Dis Model Mech 10:645-654 (2017). PubMed: 28213588
  • Kim Wiese A  et al. DNA-PKcs controls calcineurin mediated IL-2 production in T lymphocytes. PLoS One 12:e0181608 (2017). PubMed: 28750002
  • Giladi M  et al. Tumor treating fields (TTFields) delay DNA damage repair following radiation treatment of glioma cells. Radiat Oncol 12:206 (2017). WB ; Human . PubMed: 29284495
  • Espana-Agusti J  et al. Loss of PBRM1 rescues VHL dependent replication stress to promote renal carcinogenesis. Nat Commun 8:2026 (2017). WB ; Mouse . PubMed: 29229903
  • Morii M  et al. Src Acts as an Effector for Ku70-dependent Suppression of Apoptosis through Phosphorylation of Ku70 at Tyr-530. J Biol Chem 292:1648-1665 (2017). PubMed: 27998981
  • Mamo T  et al. Inhibiting DNA-PKCS radiosensitizes human osteosarcoma cells. Biochem Biophys Res Commun 486:307-313 (2017). PubMed: 28300555
  • Yin Y  et al. Androgen Receptor Variants Mediate DNA Repair after Prostate Cancer Irradiation. Cancer Res 77:4745-4754 (2017). PubMed: 28754673
  • Wang C  et al. ATM-Deficient Colorectal Cancer Cells Are Sensitive to the PARP Inhibitor Olaparib. Transl Oncol 10:190-196 (2017). WB . PubMed: 28182994
  • Smith EA  et al. DEK is required for homologous recombination repair of DNA breaks. Sci Rep 7:44662 (2017). WB . PubMed: 28317934
  • Natale F  et al. Identification of the elementary structural units of the DNA damage response. Nat Commun 8:15760 (2017). PubMed: 28604675
  • Bot C  et al. Independent mechanisms recruit the cohesin loader protein NIPBL to sites of DNA damage. J Cell Sci 130:1134-1146 (2017). PubMed: 28167679
  • Toulany M  et al. Dual targeting of PI3K and MEK enhances the radiation response of K-RAS mutated non-small cell lung cancer. Oncotarget 7:43746-43761 (2016). PubMed: 27248324
  • Couturier AM  et al. Roles for APRIN (PDS5B) in homologous recombination and in ovarian cancer prediction. Nucleic Acids Res 44:10879-10897 (2016). PubMed: 27924011
  • Finzel A  et al. Hyperactivation of ATM upon DNA-PKcs inhibition modulates p53 dynamics and cell fate in response to DNA damage. Mol Biol Cell 27:2360-7 (2016). WB . PubMed: 27280387
  • Vuono EA  et al. The PTEN phosphatase functions cooperatively with the Fanconi anemia proteins in DNA crosslink repair. Sci Rep 6:36439 (2016). ICC/IF ; Human . PubMed: 27819275
  • Harley ME  et al. TRAIP promotes DNA damage response during genome replication and is mutated in primordial dwarfism. Nat Genet 48:36-43 (2016). WB ; Human . PubMed: 26595769
  • Cristini A  et al. DNA-PK triggers histone ubiquitination and signaling in response to DNA double-strand breaks produced during the repair of transcription-blocking topoisomerase I lesions. Nucleic Acids Res 44:1161-78 (2016). WB ; Human . PubMed: 26578593
  • Renaud E  et al. Impaired TIP60-mediated H4K16 acetylation accounts for the aberrant chromatin accumulation of 53BP1 and RAP80 in Fanconi anemia pathway-deficient cells. Nucleic Acids Res 44:648-56 (2016). ICC/IF ; Human . PubMed: 26446986
  • Tadi SK  et al. PAXX Is an Accessory c-NHEJ Factor that Associates with Ku70 and Has Overlapping Functions with XLF. Cell Rep 17:541-555 (2016). PubMed: 27705800
  • Abu-Sanad A  et al. Simultaneous inhibition of ATR and PARP sensitizes colon cancer cell lines to irinotecan. Front Pharmacol 6:147 (2015). WB . PubMed: 26257651
  • Nakahara T  et al. Activation of NF-?B by human papillomavirus 16 E1 limits E1-dependent viral replication through degradation of E1. J Virol 89:5040-59 (2015). PubMed: 25717108
  • Wagner W  et al. L- and D-lactate enhance DNA repair and modulate the resistance of cervical carcinoma cells to anticancer drugs via histone deacetylase inhibition and hydroxycarboxylic acid receptor 1 activation. Cell Commun Signal 13:36 (2015). ICC/IF ; Human . PubMed: 26208712
  • DeLoughery Z  et al. DNA double-strand breaks by Cr(VI) are targeted to euchromatin and cause ATR-dependent phosphorylation of histone H2AX and its ubiquitination. Toxicol Sci 143:54-63 (2015). PubMed: 25288669
  • Wilson JE  et al. Inflammasome-independent role of AIM2 in suppressing colon tumorigenesis via DNA-PK and Akt. Nat Med N/A:N/A (2015). PubMed: 26107252
  • Burdine LJ  et al. Proteomic Identification of DNA-PK Involvement within the RET Signaling Pathway. PLoS One 10:e0127943 (2015). WB ; Human . PubMed: 26065416
  • Goglia AG  et al. Identification of novel radiosensitizers in a high-throughput, cell-based screen for DSB repair inhibitors. Mol Cancer Ther 14:326-42 (2015). PubMed: 25512618
  • Nakajima NI  et al. Pre-exposure to ionizing radiation stimulates DNA double strand break end resection, promoting the use of homologous recombination repair. PLoS One 10:e0122582 (2015). PubMed: 25826455
  • Maachani UB  et al. Targeting MPS1 Enhances Radiosensitization of Human Glioblastoma by Modulating DNA Repair Proteins. Mol Cancer Res 13:852-62 (2015). PubMed: 25722303
  • Bouley J  et al. A new phosphorylated form of Ku70 identified in resistant leukemic cells confers fast but unfaithful DNA repair in cancer cell lines. Oncotarget 6:27980-8000 (2015). PubMed: 26337656
  • Guo Y  et al. ATM-dependent Phosphorylation of the Fanconi Anemia Protein PALB2 Promotes the DNA Damage Response. J Biol Chem 290:27545-56 (2015). PubMed: 26420486
  • Gustafsson AS  et al. Suppression of DNA-dependent protein kinase sensitize cells to radiation without affecting DSB repair. Mutat Res 769:1-10 (2014). PubMed: 25771720
  • Sahlberg SH  et al. The influence of AKT isoforms on radiation sensitivity and DNA repair in colon cancer cell lines. Tumour Biol 35:3525-34 (2014). WB . PubMed: 24338765
  • Osterman M  et al. Elevated DNA damage response in pancreatic cancer. Histochem Cell Biol 142:713-20 (2014). PubMed: 25002126
  • Zhang B  et al. Mitochondria regulate DNA damage and genomic instability induced by high LET radiation. Life Sci Space Res (Amst) 1:80-88 (2014). PubMed: 25072018
  • Yuan M  et al. RNA binding protein RBM14 promotes radio-resistance in glioblastoma by regulating DNA repair and cell differentiation. Oncotarget 5:2820-6 (2014). WB . PubMed: 24811242
  • Spurlock CF  et al. Methotrexate inhibits NF-?B activity via long intergenic (noncoding) RNA-p21 induction. Arthritis Rheumatol 66:2947-57 (2014). PubMed: 25077978
  • Al-Mashhadi S  et al. Oxidative Glial Cell Damage Associated with White Matter Lesions in the Aging Human Brain. Brain Pathol N/A:N/A (2014). WB ; Human . PubMed: 25311358
  • García CP  et al. Topoisomerase I inhibitor, camptothecin, induces apoptogenic signaling in human embryonic stem cells. Stem Cell Res 12:400-14 (2014). ICC/IF ; Human . PubMed: 24380814
  • Zhou Y  et al. Quantitation of DNA double-strand break resection intermediates in human cells. Nucleic Acids Res 42:e19 (2014). ChIP ; Human . PubMed: 24362840
  • Yu H  et al. Tumor suppressor and deubiquitinase BAP1 promotes DNA double-strand break repair. Proc Natl Acad Sci U S A 111:285-90 (2014). PubMed: 24347639
  • Gad H  et al. MTH1 inhibition eradicates cancer by preventing sanitation of the dNTP pool. Nature 508:215-21 (2014). PubMed: 24695224
  • Peng M  et al. Crosstalk between BRCA-Fanconi anemia and mismatch repair pathways prevents MSH2-dependent aberrant DNA damage responses. EMBO J N/A:N/A (2014). WB, ICC/IF ; Human . PubMed: 24966277
  • Neal JA  et al. Unraveling the Complexities of DNA-Dependent Protein Kinase Autophosphorylation. Mol Cell Biol 34:2162-75 (2014). WB . PubMed: 24687855
  • Montano R  et al. Sensitization of human cancer cells to gemcitabine by the Chk1 inhibitor MK-8776: cell cycle perturbation and impact of administration schedule in vitro and in vivo. BMC Cancer 13:604 (2013). WB ; Human . PubMed: 24359526
  • Valerie NC  et al. Inhibition of T-type calcium channels disrupts Akt signaling and promotes apoptosis in glioblastoma cells. Biochem Pharmacol 85:888-97 (2013). PubMed: 23287412
  • Calkins AS  et al. DNA damage-induced inhibition of rRNA synthesis by DNA-PK and PARP-1. Nucleic Acids Res 41:7378-86 (2013). PubMed: 23775790
  • Shawi M  et al. Telomerase contributes to fludarabine resistance in primary human leukemic lymphocytes. PLoS One 8:e70428 (2013). Flow Cyt ; Human . PubMed: 23922990
  • Chatterjee P  et al. Defective chromatin recruitment and retention of NHEJ core components in human tumor cells expressing a Cyclin E fragment. Nucleic Acids Res N/A:N/A (2013). PubMed: 24021630
  • Tu WZ  et al. ?H2AX foci formation in the absence of DNA damage: Mitotic H2AX phosphorylation is mediated by the DNA-PKcs/CHK2 pathway. FEBS Lett 587:3437-43 (2013). PubMed: 24021642
  • Yajima H  et al. The complexity of DNA double strand breaks is a critical factor enhancing end-resection. DNA Repair (Amst) 12:936-46 (2013). PubMed: 24041488
  • Boisvert RA  et al. Coordinate Nuclear Targeting of the FANCD2 and FANCI Proteins via a FANCD2 Nuclear Localization Signal. PLoS One 8:e81387 (2013). ICC/IF . PubMed: 24278431
  • Zhou Y & Paull TT DNA-dependent protein kinase regulates DNA end resection in concert with Mre11-Rad50-Nbs1 (MRN) and ataxia telangiectasia-mutated (ATM). J Biol Chem 288:37112-25 (2013). Human . PubMed: 24220101
  • Lundholm L  et al. Resistance to DNA-damaging treatment in non-small cell lung cancer tumor-initiating cells involves reduced DNA-PK/ATM activation and diminished cell cycle arrest. Cell Death Dis 4:e478 (2013). WB ; Human . PubMed: 23370278
  • Chitnis MM  et al. IGF-1R inhibition enhances radiosensitivity and delays double-strand break repair by both non-homologous end-joining and homologous recombination. Oncogene : (2013). PubMed: 24186206
  • Žumer K  et al. Unmodified histone H3K4 and DNA-dependent protein kinase recruit autoimmune regulator to target genes. Mol Cell Biol 32:1354-62 (2012). PubMed: 22310661
  • Gandhi M  et al. Homologous chromosomes make contact at the sites of double-strand breaks in genes in somatic G0/G1-phase human cells. Proc Natl Acad Sci U S A 109:9454-9 (2012). PubMed: 22645362
  • Noda A  et al. Unrepairable DNA double-strand breaks that are generated by ionising radiation determine the fate of normal human cells. J Cell Sci 125:5280-7 (2012). PubMed: 22899723
  • Mauro M  et al. p21 promotes error-free replication-coupled DNA double-strand break repair. Nucleic Acids Res 40:8348-60 (2012). WB . PubMed: 22735704
  • Dean JL  et al. Modification of the DNA Damage Response by Therapeutic CDK4/6 Inhibition. J Biol Chem 287:29075-87 (2012). WB . PubMed: 22733811
  • Meek K  et al. N-terminal constraint activates the catalytic subunit of the DNA-dependent protein kinase in the absence of DNA or Ku. Nucleic Acids Res 40:2964-73 (2012). WB . PubMed: 22167471
  • Furusawa Y  et al. DNA double-strand breaks induced by cavitational mechanical effects of ultrasound in cancer cell lines. PLoS One 7:e29012 (2012). ICC/IF ; Human . PubMed: 22235259
  • Kang MA  et al. Deregulation of DNA damage response pathway by intercellular contact. J Biol Chem 287:16246-55 (2012). WB . PubMed: 22431734
  • Bouquet F  et al. A DNA-dependent stress response involving DNA-PK occurs in hypoxic cells and contributes to cellular adaptation to hypoxia. J Cell Sci 124:1943-51 (2011). PubMed: 21576354
  • Lossaint G  et al. Chk1 is dispensable for G2 arrest in response to sustained DNA damage when the ATM/p53/p21 pathway is functional. Oncogene 30:4261-74 (2011). PubMed: 21532626
  • Ogiwara H  et al. Histone acetylation by CBP and p300 at double-strand break sites facilitates SWI/SNF chromatin remodeling and the recruitment of non-homologous end joining factors. Oncogene 30:2135-46 (2011). PubMed: 21217779
  • Adamo A  et al. Preventing nonhomologous end joining suppresses DNA repair defects of Fanconi anemia. Mol Cell 39:25-35 (2010). PubMed: 20598602
  • Du L  et al. Radiosensitization and growth inhibition of cancer cells mediated by an scFv antibody gene against DNA-PKcs in vitro and in vivo. Radiat Oncol 5:70 (2010). PubMed: 20704701
  • Karimi-Busheri F  et al. Senescence evasion by MCF-7 human breast tumor-initiating cells. Breast Cancer Res 12:R31 (2010). WB, IHC . PubMed: 20525204
  • Sakasai R  et al. Proteasome inhibition suppresses DNA-dependent protein kinase activation caused by camptothecin. DNA Repair (Amst) 9:76-82 (2010). PubMed: 19959400
  • Velkova A  et al. Identification of filamin A as a BRCA1-interacting protein required for efficient DNA repair. Cell Cycle 9: (2010). WB ; Human . PubMed: 20305393
  • Shang ZF  et al. Inactivation of DNA-dependent protein kinase leads to spindle disruption and mitotic catastrophe with attenuated checkpoint protein 2 Phosphorylation in response to DNA damage. Cancer Res 70:3657-66 (2010). WB ; Human . PubMed: 20406977
  • Sakasai R  et al. Transcription-dependent activation of ataxia telangiectasia mutated prevents DNA-dependent protein kinase-mediated cell death in response to topoisomerase I poison. J Biol Chem 285:15201-8 (2010). WB ; Human . PubMed: 20304914
  • White JS  et al. Transient ATM kinase inhibition disrupts DNA damage-induced sister chromatid exchange. Sci Signal 3:ra44 (2010). PubMed: 20516478
  • Brugat T  et al. Telomere dysfunction-induced foci arise with the onset of telomeric deletions and complex chromosomal aberrations in resistant chronic lymphocytic leukemia cells. Blood 116:239-49 (2010). ICC/IF ; Human . PubMed: 20424183
  • Zhu H  et al. Involvement of Caveolin-1 in repair of DNA damage through both homologous recombination and non-homologous end joining. PLoS One 5: (2010). WB ; Human . PubMed: 20700465
  • Massip L  et al. Deciphering the chromatin landscape induced around DNA double strand breaks. Cell Cycle 9:2963-72 (2010). ICC/IF, ChIP ; Human . PubMed: 20714222
  • Bensimon A  et al. ATM-dependent and -independent dynamics of the nuclear phosphoproteome after DNA damage. Sci Signal 3:rs3 (2010). WB ; Human . PubMed: 21139141
  • Marston E  et al. Stratification of pediatric ALL by in vitro cellular responses to DNA double-strand breaks provides insight into the molecular mechanisms underlying clinical response. Blood 113:117-26 (2009). WB ; Human . PubMed: 18941120
  • Solier S  et al. Death receptor-induced activation of the Chk2- and histone H2AX-associated DNA damage response pathways. Mol Cell Biol 29:68-82 (2009). WB ; Human . PubMed: 18955500
  • Tsai CY  et al. Targeting DNA repair in chronic lymphocytic leukemia cells with a novel acyclic nucleotide analogue, GS-9219. Clin Cancer Res 15:3760-9 (2009). WB ; Human . PubMed: 19435836
  • Tomimatsu N  et al. Distinct roles of ATR and DNA-PKcs in triggering DNA damage responses in ATM-deficient cells. EMBO Rep 10:629-35 (2009). PubMed: 19444312
  • Mordes DA  et al. TopBP1 activates ATR through ATRIP and a PIKK regulatory domain. Genes Dev 22:1478-89 (2008). WB . PubMed: 18519640
  • Willmore E  et al. DNA-Dependent Protein Kinase Is a Therapeutic Target and an Indicator of Poor Prognosis in B-Cell Chronic Lymphocytic Leukemia. Clin Cancer Res 14:3984-3992 (2008). WB, Flow Cyt ; Human . PubMed: 18559621
  • Douglas P  et al. The DNA-dependent protein kinase catalytic subunit is phosphorylated in vivo on threonine 3950, a highly conserved amino acid in the protein kinase domain. Mol Cell Biol 27:1581-91 (2007). WB ; Human . PubMed: 17158925

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