For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    dna-pkcs-phospho-s2056-antibody-epr5670-bsa-and-azide-free-ab174576.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cell Biology Apoptosis Nucleus Other
Share by email
RecombinantRabMAb

Recombinant Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)

  • Datasheet
  • SDS
Submit a review Submit a question References (2)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

ELISA - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)
  • Immunocytochemistry/ Immunofluorescence - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)
  • OI-RD Scanning - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)
  • Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5670] to DNA PKcs (phospho S2056) - BSA and Azide free
  • Suitable for: IHC-P, WB, ELISA, ICC/IF
  • Reacts with: Human

You may also be interested in

ELISA
Product image
Human MMP13 ELISA Kit (ab100605)
Primary
Product image
Anti-Alpha Dystroglycan antibody [45-3] (ab199768)
Conjugation
Product image
Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link® (ab236553)

View more associated products

Overview

  • Product name

    Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free
    See all DNA PKcs primary antibodies
  • Description

    Rabbit monoclonal [EPR5670] to DNA PKcs (phospho S2056) - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab174576 is the carrier-free version of ab124918 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab174576 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Protein-A purification via MabSelect SuRe
  • Clonality

    Monoclonal
  • Clone number

    EPR5670
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Nucleus
    • Other
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Non Homol. End Joining
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • DNA Damage Recognition
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • Other
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Cancer
    • Cell Death
    • Apoptosis
    • Nucleus
    • Other

Associated products

  • Alternative Versions

    • Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab174576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB
Use at an assay dependent concentration. Predicted molecular weight: 469 kDa.
ELISA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB
Use at an assay dependent concentration. Predicted molecular weight: 469 kDa.
ELISA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.

Target

  • Function

    Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D.
  • Sequence similarities

    Belongs to the PI3/PI4-kinase family.
    Contains 1 FAT domain.
    Contains 1 FATC domain.
    Contains 2 HEAT repeats.
    Contains 1 PI3K/PI4K domain.
    Contains 3 TPR repeats.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR. Autophosphorylated on Thr-2609, Thr-2638 and Thr-2647. Thr-2609 is a DNA damage-inducible phosphorylation site (inducible with ionizing radiation, IR). Autophosphorylation induces a conformational change that leads to remodeling of the DNA-PK complex, requisite for efficient end processing and DNA repair.
    S-nitrosylated by GAPDH.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P78527 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5591 Human
    • Omim: 600899 Human
    • SwissProt: P78527 Human
    • Unigene: 491682 Human
    • Alternative names

      • DNA dependent protein kinase catalytic subunit antibody
      • DNA PK catalytic subunit antibody
      • DNA-dependent protein kinase catalytic subunit antibody
      • DNA-PK catalytic subunit antibody
      • DNA-PKcs antibody
      • DNAPK antibody
      • DNAPK catalytic subunit antibody
      • DNPK 1 antibody
      • DNPK1 antibody
      • Hyper radiosensitivity of murine scid mutation, complementing 1 antibody
      • Hyperradiosensitivity complementing 1, mouse, homolog of 1 antibody
      • HYRC 1 antibody
      • HYRC antibody
      • HYRC1 antibody
      • IMD26 antibody
      • p350 antibody
      • p460 antibody
      • PKRDC antibody
      • PRKDC antibody
      • PRKDC_HUMAN antibody
      • Protein Kinase DNA Activated Catalytic Polypeptide antibody
      • XRCC 7 antibody
      • XRCC7 antibody
      see all

    Images

    • ELISA - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)
      ELISA - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)

      Serially diluted ab124918 was bound to immobilised phospho- or control peptides (1 microgram per mL). The antibody was detected by HRP-labelled goat anti-rabbit IgG (ab97080; diluted 50000 times) and signal was developed with TMB substrate.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124918).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling DNA PKcs (phospho S2056) with purified ab124918 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124918).

    • Immunocytochemistry/ Immunofluorescence - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)
      Immunocytochemistry/ Immunofluorescence - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)

      Immunocytochemistry/Immunofluorescence analysis of Jurkat cells (untreated and treated with Alkaline Phosphatase) labelling DNA PKcs (phospho S2056) with purified ab124918 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

      Control 1: primary antibody (1/500) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

      Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124918).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling DNA PKcs (phospho S2056) with unpurified ab124918 at a dilution of 1/100.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124918).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • OI-RD Scanning - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)
      OI-RD Scanning - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124918).

    • Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)
      Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab174576? Please let us know so that we can cite the reference in this datasheet.

    ab174576 has been referenced in 2 publications.

    • Xie Y  et al. RBX1 prompts degradation of EXO1 to limit the homologous recombination pathway of DNA double-strand break repair in G1 phase. Cell Death Differ 27:1383-1397 (2020). PubMed: 31562368
    • El Bezawy R  et al. miR-205 enhances radiation sensitivity of prostate cancer cells by impairing DNA damage repair through PKCe and ZEB1 inhibition. J Exp Clin Cancer Res 38:51 (2019). PubMed: 30717752

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab174576.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.