Anti-DNA polymerase alpha/POLA antibody (ab176734)
Overview
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Product name
Anti-DNA polymerase alpha/POLA antibody
See all DNA polymerase alpha/POLA primary antibodies -
Description
Rabbit polyclonal to DNA polymerase alpha/POLA -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Horse, Chicken, Guinea pig, Dog, Non human primates
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Immunogen
Synthetic peptide within Human DNA polymerase alpha/POLA aa 1175-1225. The exact sequence is proprietary. NP_058633.2.
Database link: P09884 -
Positive control
- HeLa and 293T cell lysates
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General notes
This product was previously labelled as DNA polymerase alpha
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab176734 is affinity purified using an epitope specific to DNA polymerase alpha/POLA immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab176734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 166 kDa. | |
| IP | Use at 2-5 µg/mg of lysate. |
Target
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Function
Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. -
Sequence similarities
Belongs to the DNA polymerase type-B family. -
Post-translational
modificationsA 165 kDa form is probably produced by proteolytic cleavage at Lys-124. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 418593 Chicken
- Entrez Gene: 480869 Dog
- Entrez Gene: 100717633 Guinea pig
- Entrez Gene: 100052225 Horse
- Entrez Gene: 5422 Human
- Entrez Gene: 100354472 Rabbit
- Omim: 312040 Human
- SwissProt: P09884 Human
see all -
Alternative names
- DKFZp686K1672 antibody
- DNA polymerase alpha 1 catalytic subunit antibody
- DNA polymerase alpha catalytic subunit antibody
see all
Images
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Western blot - Anti-DNA polymerase alpha/POLA antibody (ab176734)All lanes : Anti-DNA polymerase alpha/POLA antibody (ab176734) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 166 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-DNA polymerase alpha/POLA antibody (ab176734)ab176734 at 0.4 µg/ml detecting DNA polymerase alpha/POLA in HeLa whole cell lysate by WB following IP. ab176734 was used for IP at 3 µg/mg of lysate, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Protocols
Datasheets and documents
References
ab176734 has been referenced in 2 publications.
- Coulombe P et al. The ORC ubiquitin ligase OBI1 promotes DNA replication origin firing. Nat Commun 10:2426 (2019). PubMed: 31160578
- Moiseeva T et al. ATR kinase inhibition induces unscheduled origin firing through a Cdc7-dependent association between GINS and And-1. Nat Commun 8:1392 (2017). PubMed: 29123096
