Product nameAnti-DNA polymerase alpha/POLA antibody
See all DNA polymerase alpha/POLA primary antibodies
DescriptionRabbit polyclonal to DNA polymerase alpha/POLA
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse, Chicken, Guinea pig, Dog, Non human primates
Synthetic peptide within Human DNA polymerase alpha/POLA aa 1175-1225. The exact sequence is proprietary. NP_058633.2.
Database link: P09884
- HeLa and 293T cell lysates
This product was previously labelled as DNA polymerase alpha
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab176734 is affinity purified using an epitope specific to DNA polymerase alpha/POLA immobilized on solid support.
Our Abpromise guarantee covers the use of ab176734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 166 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionPlays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.
Sequence similaritiesBelongs to the DNA polymerase type-B family.
modificationsA 165 kDa form is probably produced by proteolytic cleavage at Lys-124.
- Information by UniProt
- DKFZp686K1672 antibody
- DNA polymerase alpha 1 catalytic subunit antibody
- DNA polymerase alpha catalytic subunit antibody
All lanes : Anti-DNA polymerase alpha/POLA antibody (ab176734) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 166 kDa
Exposure time: 3 minutes
ab176734 at 0.4 µg/ml detecting DNA polymerase alpha/POLA in HeLa whole cell lysate by WB following IP. ab176734 was used for IP at 3 µg/mg of lysate, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 10 seconds.
This product has been referenced in:
- Coulombe P et al. The ORC ubiquitin ligase OBI1 promotes DNA replication origin firing. Nat Commun 10:2426 (2019). Read more (PubMed: 31160578) »
- Moiseeva T et al. ATR kinase inhibition induces unscheduled origin firing through a Cdc7-dependent association between GINS and And-1. Nat Commun 8:1392 (2017). Read more (PubMed: 29123096) »