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Epigenetics and Nuclear Signaling DNA / RNA DNA Synthesis DNA Polymerases
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Anti-DNA polymerase alpha/POLA antibody (ab176734)

  • Datasheet
  • SDS
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Western blot - Anti-DNA polymerase alpha/POLA antibody (ab176734)
  • Immunoprecipitation - Anti-DNA polymerase alpha/POLA antibody (ab176734)

Key features and details

  • Rabbit polyclonal to DNA polymerase alpha/POLA
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human DNA polymerase alpha/POLA protein (ab114839)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-DNA polymerase alpha/POLA antibody
    See all DNA polymerase alpha/POLA primary antibodies
  • Description

    Rabbit polyclonal to DNA polymerase alpha/POLA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Chicken, Guinea pig, Dog, Non human primates
  • Immunogen

    Synthetic peptide within Human DNA polymerase alpha/POLA aa 1175-1225. The exact sequence is proprietary. NP_058633.2.
    Database link: P09884

  • Positive control

    • HeLa and 293T cell lysates
  • General notes

     This product was previously labelled as DNA polymerase alpha

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, 99% Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab176734 is affinity purified using an epitope specific to DNA polymerase alpha/POLA immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • DNA Polymerases

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human DNA polymerase alpha/POLA protein (ab114839)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab176734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/2000 - 1/10000. Predicted molecular weight: 166 kDa.
IP
Use at 2-5 µg/mg of lysate.
Notes
WB
1/2000 - 1/10000. Predicted molecular weight: 166 kDa.
IP
Use at 2-5 µg/mg of lysate.

Target

  • Function

    Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.
  • Sequence similarities

    Belongs to the DNA polymerase type-B family.
  • Post-translational
    modifications

    A 165 kDa form is probably produced by proteolytic cleavage at Lys-124.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P09884 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 418593 Chicken
    • Entrez Gene: 480869 Dog
    • Entrez Gene: 100717633 Guinea pig
    • Entrez Gene: 100052225 Horse
    • Entrez Gene: 5422 Human
    • Entrez Gene: 100354472 Rabbit
    • Omim: 312040 Human
    • SwissProt: P09884 Human
    • Unigene: 567319 Human
    see all
  • Alternative names

    • DKFZp686K1672 antibody
    • DNA polymerase alpha 1 catalytic subunit antibody
    • DNA polymerase alpha catalytic subunit antibody
    • DNA polymerase alpha catalytic subunit p180 antibody
    • DNA polymerase alpha p180 subunit antibody
    • DPOLA_HUMAN antibody
    • OTTHUMP00000023089 antibody
    • OTTHUMP00000023090 antibody
    • P180 antibody
    • p180 subunit antibody
    • POLA antibody
    • POLA1 antibody
    • Polymerase (DNA directed), alpha 1, catalytic subunit antibody
    • Polymerase (DNA-directed), alpha (70kD) antibody
    see all

Images

  • Western blot - Anti-DNA polymerase alpha/POLA antibody (ab176734)
    Western blot - Anti-DNA polymerase alpha/POLA antibody (ab176734)
    All lanes : Anti-DNA polymerase alpha/POLA antibody (ab176734) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 166 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation - Anti-DNA polymerase alpha/POLA antibody (ab176734)
    Immunoprecipitation - Anti-DNA polymerase alpha/POLA antibody (ab176734)

    ab176734 at 0.4 µg/ml detecting DNA polymerase alpha/POLA in HeLa whole cell lysate by WB following IP.  ab176734 was used for IP at 3 µg/mg of lysate, 1 mg of lysate was used for IP and 20% of the IP was loaded.

    Detection: Chemiluminescence with an exposure time of 10 seconds.

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab176734? Please let us know so that we can cite the reference in this datasheet.

ab176734 has been referenced in 2 publications.

  • Coulombe P  et al. The ORC ubiquitin ligase OBI1 promotes DNA replication origin firing. Nat Commun 10:2426 (2019). PubMed: 31160578
  • Moiseeva T  et al. ATR kinase inhibition induces unscheduled origin firing through a Cdc7-dependent association between GINS and And-1. Nat Commun 8:1392 (2017). PubMed: 29123096

Customer reviews and Q&As

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