Product nameAnti-DNA polymerase alpha/POLA antibody
See all DNA polymerase alpha/POLA primary antibodies
DescriptionRabbit polyclonal to DNA polymerase alpha/POLA
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Chicken, Human, Xenopus laevis
Predicted to work with: Mouse, Rat, Dog
Synthetic peptide corresponding to Human DNA polymerase alpha/POLA aa 1100-1200.
(Peptide available as
- This antibody gave a positive signal in the following whole cell lysates: (WB) HeLa; Jurkat; A431: (IF) MCF7; HeLa; HEK293; HepG2
This product was previously labelled as DNA polymerase alpha
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab31777 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 166 kDa).|
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionPlays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.
Sequence similaritiesBelongs to the DNA polymerase type-B family.
modificationsA 165 kDa form is probably produced by proteolytic cleavage at Lys-124.
- Information by UniProt
- DKFZp686K1672 antibody
- DNA polymerase alpha 1 catalytic subunit antibody
- DNA polymerase alpha catalytic subunit antibody
All lanes : Anti-DNA polymerase alpha/POLA antibody (ab31777) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :
Jurkat whole cell lysate (ab7899)
Lane 3 :
A431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 166 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Image courtesy of Human Protein Atlas ab31777 staining DNA polymerase alpha/POLA in human thyroid gland, showing a strong staining in nuclei of the glandular cells. Paraffin embedded human thyroid tissue was incubated with ab31777 (1/100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab31777 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
This product has been referenced in:
- Abdel-Samad R et al. Mechanism of action of the atypical retinoid ST1926 in colorectal cancer: DNA damage and DNA polymerase a. Am J Cancer Res 8:39-55 (2018). Read more (PubMed: 29416919) »
- Bellelli R et al. Pole Instability Drives Replication Stress, Abnormal Development, and Tumorigenesis. Mol Cell 70:707-721.e7 (2018). Read more (PubMed: 29754823) »