Key features and details
- Rabbit polyclonal to DNA polymerase alpha/POLA
- Suitable for: WB
- Reacts with: Chicken, Human
- Isotype: IgG
Product nameAnti-DNA polymerase alpha/POLA antibody
See all DNA polymerase alpha/POLA primary antibodies
DescriptionRabbit polyclonal to DNA polymerase alpha/POLA
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Chicken, Human
Predicted to work with: Mouse, Rat, Dog, Xenopus laevis
Synthetic peptide corresponding to Human DNA polymerase alpha/POLA aa 1100-1200.
(Peptide available as
This product was previously labelled as DNA polymerase alpha
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab31777 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 166 kDa).|
FunctionPlays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.
Sequence similaritiesBelongs to the DNA polymerase type-B family.
modificationsA 165 kDa form is probably produced by proteolytic cleavage at Lys-124.
- Information by UniProt
- DKFZp686K1672 antibody
- DNA polymerase alpha 1 catalytic subunit antibody
- DNA polymerase alpha catalytic subunit antibody
All lanes : Anti-DNA polymerase alpha/POLA antibody (ab31777) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :
Jurkat whole cell lysate (ab7899)
Lane 3 :
A431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 166 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
ab31777 has been referenced in 7 publications.
- Abdel-Samad R et al. Mechanism of action of the atypical retinoid ST1926 in colorectal cancer: DNA damage and DNA polymerase a. Am J Cancer Res 8:39-55 (2018). PubMed: 29416919
- Bellelli R et al. Pole Instability Drives Replication Stress, Abnormal Development, and Tumorigenesis. Mol Cell 70:707-721.e7 (2018). PubMed: 29754823
- Kolinjivadi AM et al. Smarcal1-Mediated Fork Reversal Triggers Mre11-Dependent Degradation of Nascent DNA in the Absence of Brca2 and Stable Rad51 Nucleofilaments. Mol Cell 67:867-881.e7 (2017). PubMed: 28757209
- Aze A et al. RNAs coordinate nuclear envelope assembly and DNA replication through ELYS recruitment to chromatin. Nat Commun 8:2130 (2017). PubMed: 29242643
- Yun J et al. Reduced cohesin destabilizes high-level gene amplification by disrupting pre-replication complex bindings in human cancers with chromosomal instability. Nucleic Acids Res 44:558-72 (2016). PubMed: 26420833
- Hashimoto Y et al. Rad51 protects nascent DNA from Mre11-dependent degradation and promotes continuous DNA synthesis. Nat Struct Mol Biol 17:1305-11 (2010). PubMed: 20935632
- Ferguson RL & Maller JL Centrosomal localization of cyclin E-Cdk2 is required for initiation of DNA synthesis. Curr Biol 20:856-60 (2010). WB ; Chinese hamster . PubMed: 20399658