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Thanks for your help. I would just be using the antibody for a Western of whole cell extracts from mouse embryonic stem cells.
Asked on Oct 08 2012
Thank you for your patience.
For ab57296, the sequence homology of the immunogen with mouse is 83% - which is good.
The lab let me know that for ab65009, the amino acid range of the immunogen is within residues 100-300 of human DNA Polymerase alpha with UniProt accession number P09884. The alignment of the immunogen sequence with the mouse protein (P33609) gives only a 61.5% sequence identity.
We usually say that a homology of 85% or higher is best for cross-reactivity. Although the immunogen region is 50 residues, the actual epitope of the antibody binding site is only 5-10 residues. This could mean the actual homology for the epitope could be either better than 61.5% or could also be worse. This is very difficult to predict and would need to be tried out.
Since none of our Anti-DNA polymerase alpha antibody has been tested in mouse, you would be eligible for our testing discount program.
For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
The testing discount program is for customers who like to use an antibody/protein/kit on an untested species/application. You would purchase the to-be-tested product at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount in the amount of the price you paid for the to-be-tested product. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial.
Please let me know if you would like to try any of the antibody and if I should send you a discount code.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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Answered on Oct 08 2012