Product nameAnti-DNA Polymerase beta antibody 
See all DNA Polymerase beta primary antibodies
DescriptionMouse monoclonal  to DNA Polymerase beta
Tested applicationsSuitable for: WB, IHC-P, IHC-Fr, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Mouse, Human
BALB/C mice were injected with DNA polymerase beta protein.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab1831 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 38 kDa.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. (PMID 20019666).|
|Flow Cyt||Use 1µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionRepair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
Sequence similaritiesBelongs to the DNA polymerase type-X family.
DomainResidues 239-252 form a flexible loop which appears to affect the polymerase fidelity.
modificationsMethylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity.
Cellular localizationNucleus, Cytoplasm
- Information by UniProt
- DNA directed DNA polymerase beta antibody
- DNA pol beta antibody
- DNA polymerase beta antibody
All lanes : Anti-DNA Polymerase beta antibody  (ab1831)
Lane 1 : Empty vector
Lane 2 : Wild-type mouse embryonic fibroblasts
Lane 3 : G231D mutant mouse embryonic fibroblasts
Lane 4 : T292I mutant mouse embryonic fibroblasts
Lane 5 : S275N/E295K mutant mouse embryonic fibroblasts
Predicted band size: 38 kDa
The normalized level of DNA Polymerase beta is indicated below each lane
ab1831 staining human testis by IHC-P.
Overlay histogram showing Jurkat cells stained with ab1831 (red line). The cells were fixed with 4% paraformaldehyde and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1831, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This product has been referenced in:
- Ray S et al. DNA polymerase beta participates in DNA End-joining. Nucleic Acids Res 46:242-255 (2018). Read more (PubMed: 29161447) »
- Lai Y et al. Crosstalk between MSH2-MSH3 and polß promotes trinucleotide repeat expansion during base excision repair. Nat Commun 7:12465 (2016). ICC . Read more (PubMed: 27546332) »