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Synthetic peptide corresponding to Human DNA Polymerase beta aa 300 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
Our Abpromise guarantee covers the use of ab26343 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).|
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||1/150. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Formaldehyde-fixed human small cell lung cancer tissue stained for DNA Polymerase beta using ab26343 at 1/200 dilution in immunohistochemical analysis.
Image courtesy of Human Protein Atlas ab26343 staining DNA polymerase beta in Human gall bladder, showing a strong nuclear staining pattern in the columnar epithelial cells in contrast with the cells in the lamina propria. Paraffin embedded human gall bladder tissue was incubated with ab26343 (1/150 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab26343 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
ICC/IF image of ab26343 stained T47D cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab26343 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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