Recombinant Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)
![Western blot - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)](https://www.abcam.com/ps/products/175/ab175197/Images/ab175197-384461-anti-dna-polymerase-beta-antibody-epr12722b-western-blot-hela-jurkat.jpg "Western blot - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12722(B)] to DNA Polymerase beta
- Suitable for: ICC/IF, IP, IHC-P, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-DNA Polymerase beta antibody [EPR12722(B)]
See all DNA Polymerase beta primary antibodies -
Description
Rabbit monoclonal [EPR12722(B)] to DNA Polymerase beta -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, IHC-P, WBmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DNA Polymerase beta aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P06746 -
Positive control
- WB: HeLa, Jurkat, K562 and A431 cell lysates. IHC-P: Human testis and uterus tissues. ICC/IF: A431 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12722(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab175197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/50 - 1/100. | |
| IP | 1/10 - 1/100. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/1000 - 1/10000. Predicted molecular weight: 38 kDa. |
Target
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Function
Repair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity. -
Sequence similarities
Belongs to the DNA polymerase type-X family. -
Domain
Residues 239-252 form a flexible loop which appears to affect the polymerase fidelity. -
Post-translational
modificationsMethylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity. -
Cellular localization
Nucleus, Cytoplasm - Information by UniProt
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Database links
- Entrez Gene: 5423 Human
- Entrez Gene: 18970 Mouse
- Entrez Gene: 29240 Rat
- Omim: 174760 Human
- SwissProt: P06746 Human
- SwissProt: Q8K409 Mouse
- SwissProt: P06766 Rat
- Unigene: 654484 Human
see all -
Alternative names
- DNA directed DNA polymerase beta antibody
- DNA pol beta antibody
- DNA polymerase beta antibody
see all
Images
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Western blot - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)All lanes : Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : POLB knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 38 kDaanes 1-3: Merged signal (red and green). Green - ab175197 observed at 38 kDa. Red - loading control, ab7291 observed at 50 kDa.
ab175197 Anti-DNA Polymerase beta antibody [EPR12722(B)] was shown to specifically react with DNA Polymerase beta in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261748 (knockout cell lysate ab257096) was used. Wild-type and DNA Polymerase beta knockout samples were subjected to SDS-PAGE. ab175197 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti- Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti- Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)](https://www.abcam.com/ps/products/175/ab175197/Images/ab175197-201547-anti-dna-polymerase-beta-antibody-epr12722b-western-blot.jpg)
Western blot - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)All lanes : Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197) at 1/1000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : Jurkat cell lysates
Lane 3 : K562 cell lysates
Lane 4 : A431 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 38 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)](https://www.abcam.com/ps/products/175/ab175197/Images/ab175197-201546-anti-dna-polymerase-beta-antibody-epr12722b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling DNA Polymerase beta with ab175197 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)](https://www.abcam.com/ps/products/175/ab175197/Images/ab175197-201545-anti-dna-polymerase-beta-antibody-epr12722b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling DNA Polymerase beta with ab175197 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)](https://www.abcam.com/ps/products/175/ab175197/Images/ab175197-201544-anti-dna-polymerase-beta-antibody-epr12722b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)Immunofluorescence analysis of A431 cells labeling DNA Polymerase beta with ab175197 at a 1/50 dilution.
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![Immunoprecipitation - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)](https://www.abcam.com/ps/products/175/ab175197/Images/ab175197-201543-anti-dna-polymerase-beta-antibody-epr12722b-immunoprecipitation.jpg)
Immunoprecipitation - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)Western blot analysis on immunoprecipitation pellet from K562 cell lysate labeling DNA Polymerase beta with ab175197 at 1/10 dilution.
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![Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)](https://www.abcam.com/ps/products/175/ab175197/Images/ab175197-7-benefits-of-recombinant-antibodies.png)
Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)
Protocols
Datasheets and documents
References (4)
ab175197 has been referenced in 4 publications.
- Jiang Z et al. Oxidative DNA Damage Modulates DNA Methylation Pattern in Human Breast Cancer 1 (BRCA1) Gene via the Crosstalk between DNA Polymerase ß and a de novo DNA Methyltransferase. Cells 9:N/A (2020). PubMed: 31963223
- Goetze RW et al. A CRISPR/Cas9 approach reveals that the polymerase activity of DNA polymerase ß is dispensable for HIV-1 infection in dividing and nondividing cells. J Biol Chem 292:14016-14025 (2017). PubMed: 28684413
- Lai Y et al. Crosstalk between MSH2-MSH3 and polß promotes trinucleotide repeat expansion during base excision repair. Nat Commun 7:12465 (2016). PubMed: 27546332
- Wang H et al. Cell cycle regulation of DNA polymerase beta in rotenone-based Parkinson's disease models. PLoS One 9:e109697 (2014). Human . PubMed: 25303312
