Recombinant Anti-DNA Polymerase beta antibody [EPR12722(B)] - BSA and Azide free (ab249852)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12722(B)] to DNA Polymerase beta - BSA and Azide free
- Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-DNA Polymerase beta antibody [EPR12722(B)] - BSA and Azide free
See all DNA Polymerase beta primary antibodies -
Description
Rabbit monoclonal [EPR12722(B)] to DNA Polymerase beta - BSA and Azide free -
Host species
Rabbit -
Specificity
The human recommendation is based on the WB results. We do not guarantee IHC-P for human.
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Tested applications
Suitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, Jurkat, K-562, A431, Mouse brain lysate, mouse spleen lysate, rat brain lysate, rat spleen lysate, C6 cell lysate ICC/IF: K-562 (Human chronic myelogenous leukemia lymphoblast) IHC: human lung carcinoma
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General notes
ab249852 is the carrier-free version of ab175197.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12722(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)
- PE Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab306093)
- APC Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab306094)
- HRP Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab306095)
- Alexa Fluor® 488 Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab309921)
- Alexa Fluor® 647 Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab310289)
- Alexa Fluor® 594 Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab310750)
- Alexa Fluor® 555 Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab312277)
- Alexa Fluor® 568 Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab312771)
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249852 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 38 kDa.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
The human recommendation is based on the WB results. We do not guarantee IHC-P for human. |
|
ICC/IF |
Use at an assay dependent concentration.
|
Notes |
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Flow Cyt
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 38 kDa. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. The human recommendation is based on the WB results. We do not guarantee IHC-P for human. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Repair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity. -
Sequence similarities
Belongs to the DNA polymerase type-X family. -
Domain
Residues 239-252 form a flexible loop which appears to affect the polymerase fidelity. -
Post-translational
modificationsMethylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity. -
Cellular localization
Nucleus, Cytoplasm - Information by UniProt
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Database links
- Entrez Gene: 5423 Human
- Entrez Gene: 18970 Mouse
- Entrez Gene: 29240 Rat
- Omim: 174760 Human
- SwissProt: P06746 Human
- SwissProt: Q8K409 Mouse
- SwissProt: P06766 Rat
- Unigene: 654484 Human
see all -
Alternative names
- DNA directed DNA polymerase beta antibody
- DNA pol beta antibody
- DNA polymerase beta antibody
see all
Images
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Lanes 1-7 : Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197) at 1/1000 dilution (Purified)
Lanes 8-9 : Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197) at 1/1000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 3 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 4 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 5 : Mouse brain lysate
Lane 6 : Mouse spleen lysate
Lane 7 : Rat brain lysate
Lane 8 : Rat spleen lysate
Lane 9 : C6 (Rat glial tumor glial cell) whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa -
This data was developed using ab175197, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling DNA Polymerase beta with Purified ab175197 at 1:50 dilution (3.1 ?g/ml). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. -
This data was developed using ab249852, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling DNA Polymerase beta with Purified ab249852 at 1:500 dilution (0.31 ?g/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. M3 -
This data was developed using ab249852, the same antibody clone in a different buffer formulation.
Flow Cytometry analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labelling DNA Polymerase beta with Purified ab249852 at 1:200 dilution (1 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor™ 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab249852 has not yet been referenced specifically in any publications.