Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12722(B)] to DNA Polymerase beta - BSA and Azide free
- Suitable for: WB, IP, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
Product nameAnti-DNA Polymerase beta antibody [EPR12722(B)] - BSA and Azide free
See all DNA Polymerase beta primary antibodies
DescriptionRabbit monoclonal [EPR12722(B)] to DNA Polymerase beta - BSA and Azide free
Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human DNA Polymerase beta aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P06746
- WB: Jurkat and HeLa cell lysates.
Ab249852 is the carrier-free version of ab175197. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab249852 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferConstituent: PBS
Concentration information loading...
KO cell lines
KO cell lysates
Our Abpromise guarantee covers the use of ab249852 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 38 kDa.|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionRepair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
Sequence similaritiesBelongs to the DNA polymerase type-X family.
DomainResidues 239-252 form a flexible loop which appears to affect the polymerase fidelity.
modificationsMethylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity.
Cellular localizationNucleus, Cytoplasm
- Information by UniProt
- DNA directed DNA polymerase beta antibody
- DNA pol beta antibody
- DNA polymerase beta antibody
All lanes : Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : POLB knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 38 kDa
This data was developed using the same antibody clone in a different buffer formulation (ab175197).
ab175197 Anti-DNA Polymerase beta antibody [EPR12722(B)] was shown to specifically react with DNA Polymerase beta in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261748 (knockout cell lysate ab257096) was used. Wild-type and DNA Polymerase beta knockout samples were subjected to SDS-PAGE. ab175197 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti- Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti- Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab249852 has not yet been referenced specifically in any publications.