Key features and details
- Rabbit polyclonal to DNA polymerase eta
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DNA polymerase eta antibody
See all DNA polymerase eta primary antibodies
DescriptionRabbit polyclonal to DNA polymerase eta
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human DNA polymerase eta aa 408-609.
TSGIQTEWSPPLTMLFLCATKFSASAPSSSTDITSFLSSDPSSLPKVPVT SSEAKTQGSGPAVTATKKATTSLESFFQKAAERQKVKEASLSSLTAPTQA PMSNSPSKPSLPFQTSQSTGTEPFFKQKSLLLKQKQLNNSSVSSPQQNPW SNCKALPNSLPTEYPGCVPVCEGVSKLEESSKATPAEMDLAHNSQSMHAS SA
Database link: Q9Y253
- WB: A549 and K562 cell lysate. IHC-P: Human tonsil tissue. ICC/IF: HepG2 cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab234855 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 78 kDa.|
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/20 - 1/200.|
FunctionDNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. May play a role in hypermutation at immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. Targets POLI to replication foci.
Involvement in diseaseDefects in POLH are the cause of xeroderma pigmentosum variant type (XPV) [MIM:278750]; also designated as XP-V. Xeroderma pigmentosum (XP) is an autosomal recessive disease due to deficient nucleotide excision repair. It is characterized by hypersensitivity of the skin to sunlight, followed by high incidence of skin cancer and frequent neurologic abnormalities. XPV shows normal nucleotide excision repair, but an exaggerated delay in recovery of replicative DNA synthesis. Most XPV patients do not develop clinical symptoms and skin neoplasias until a later age. Clinical manifestations are limited to photo-induced deterioration of the skin and eyes.
Sequence similaritiesBelongs to the DNA polymerase type-Y family.
Contains 1 umuC domain.
DomainThe catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.
Cellular localizationNucleus. Accumulates at replication forks after DNA damage.
- Information by UniProt
- DNA polymerase eta antibody
- FLJ16395 antibody
- FLJ21978 antibody
All lanes : Anti-DNA polymerase eta antibody (ab234855) at 1/500 dilution
Lane 1 : A549 (Human lung carcinoma cell line) cell lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 78 kDa
HepG2 (human liver hepatocellular carcinoma cell line) cells labeling DNA polymerase eta using ab234855 at 1/100 dilution in ICC/IF. Secondary antibody was an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (H+L).
Paraffin-embedded human tonsil tissue stained for DNA polymerase eta using ab234855 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab234855 has not yet been referenced specifically in any publications.