Overview

  • Product name

    Anti-DNA polymerase eta antibody
    See all DNA polymerase eta primary antibodies
  • Description

    Rabbit polyclonal to DNA polymerase eta
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human DNA polymerase eta (C terminal). (Around 300 amino acids of the C-terminus of the human protein).
    Database link: Q9Y253

  • Positive control

    • WB: U-2 OS, MEF and INS-1 whole cell extracts. IP: U-2 OS whole cell extract. Flow cyt: U-2 OS cells. ICC/IF: U-2 OS cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab236450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 78 kDa.
IP Use at an assay dependent concentration.

Use 3 µl to ~400 µg of extact.

ICC/IF 1/2000.
Flow Cyt 1/200.

Target

  • Function

    DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. May play a role in hypermutation at immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. Targets POLI to replication foci.
  • Involvement in disease

    Defects in POLH are the cause of xeroderma pigmentosum variant type (XPV) [MIM:278750]; also designated as XP-V. Xeroderma pigmentosum (XP) is an autosomal recessive disease due to deficient nucleotide excision repair. It is characterized by hypersensitivity of the skin to sunlight, followed by high incidence of skin cancer and frequent neurologic abnormalities. XPV shows normal nucleotide excision repair, but an exaggerated delay in recovery of replicative DNA synthesis. Most XPV patients do not develop clinical symptoms and skin neoplasias until a later age. Clinical manifestations are limited to photo-induced deterioration of the skin and eyes.
  • Sequence similarities

    Belongs to the DNA polymerase type-Y family.
    Contains 1 umuC domain.
  • Domain

    The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.
  • Cellular localization

    Nucleus. Accumulates at replication forks after DNA damage.
  • Information by UniProt
  • Database links

  • Alternative names

    • DNA polymerase eta antibody
    • FLJ16395 antibody
    • FLJ21978 antibody
    • Polh antibody
    • POLH_HUMAN antibody
    • polymerase DNA directed eta antibody
    • RAD30 antibody
    • RAD30 homolog A antibody
    • RAD30A antibody
    • Xeroderma pigmentosum variant type protein antibody
    • XP V antibody
    • XPV antibody
    see all

Images

  • All lanes : Anti-DNA polymerase eta antibody (ab236450) at 1/2000 dilution

    Lane 1 : U-2 OS (human bone osteosarcoma epithelial cell line) treated with control siRNA, whole cell lysate
    Lane 2 : U-2 OS treated with DNA polymerase eta siRNA, whole cell lysate
    Lane 3 : MEF (mouse embryonic fibroblast cell line) whole cell lysate
    Lane 4 : INS-1 (rat pancreatic beta cell line) whole cell lysate

    Lysates/proteins at 40 µg per lane.

    Predicted band size: 78 kDa



    Blocking buffer: 5% milk in TBS-T

    Incubated overnight with primary antibody.

  • DNA polymerase eta was immunoprecipitated from ~400 µg U-2 OS (human bone osteosarcoma epithelial cell line) whole cell extract with 3 μl ab236450. Western blot was performed from the immunoprecipitate using ab236450 at 1/2000 dilution.

    Lane 1: U-2 OS whole cell extract (Input)

    Lane 2: Control IgG IP in U-2 OS whole cell extract.

    Lane 3: ab236450 IP in U-2 OS whole cell extract.

  • Flow cytometric analysis of 1% PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cell line labeling DNA polymerase eta with ab236450 at 1/200 dilution (green) compared with a no primary antibody control (blue). 

  • U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for DNA polymerase eta (green) using ab236450 at 1/2000 dilution in ICC/IF (green). The nuclear counter stain is DAPI (blue). The right-hand panel shows the merged images.

References

ab236450 has not yet been referenced specifically in any publications.

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