Overview

  • Product name

    Anti-DNA polymerase I antibody
  • Description

    Rabbit Polyclonal to DNA polymerase I
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Escherichia coli
  • Immunogen

    Recombinant full length protein corresponding to Escherichia coli DNA polymerase I aa 1-928.
    Sequence:

    MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLNMLRSLI MQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVK AMGLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNIT LINTMTNTILGPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKT AQALLQGLGGLDTLYAEPEKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLA TIKTDVELELTCEQLEVQQPAAEELLGLFKKYEFKRWTADVEAGKWLQAK GAKPAAKPQETSVADEAPEVTATVISYDNYVTILDEETLKAWIAKLEKAP VFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDAPDQISRER ALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAE DADVTLQLHLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPK VLHNHSEELTLRLAELEKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLK KTPGGAPSTSEEVLEELALDYPLPKVILEYRGLAKLKSTYTDKLPLMINP KTGRVHTSYHQAVTATGRLSSTDPNLQNIPVRNEEGRRIRQAFIAPEDYV IVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAEVFGLPLETVT SEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGVLE YMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQG TAADIIKRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQI HQLMENCTRLDVPLLVEVGSGENWDQAH


    Database link: P00582

  • Positive control

    • Purified Escherichia coli DNA polymerase alpha; Sonic lysate supernatant of Escherichia coli strain AB1157 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab188424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB 1/1000 - 1/5000. Predicted molecular weight: 103 kDa.

Target

Images

  • All lanes : Anti-DNA polymerase I antibody (ab188424) at 1/2000 dilution

    Lane 1 : Purified Escherichia coli DNA polymerase alpha at 0.01 µg
    Lane 2 : Sonic lysate supernatant of Escherichia coli strain AB1157 cells

    Predicted band size: 103 kDa

  • ab188424 detecting DNA polymerase alpha in Escherichia coli strain AB1157 crude cell lysate was precipitated with protein G conjugated magnetic beads and analyzed by WB by using ab188424 antibody.
    ab 131366 VeriBlot for IP secondary antibody (HRP) was used for secondary staining.

References

ab188424 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab188424.
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