Product nameAnti-DNA Polymerase iota antibody
See all DNA Polymerase iota primary antibodies
DescriptionRabbit polyclonal to DNA Polymerase iota
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Monkey, Gorilla
Synthetic peptide aa 690-715. The exact sequence is proprietary.
Database link: Q9UNA4
- WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264193 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionError-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.
Tissue specificityUbiquitous. Highly expressed in testis.
Sequence similaritiesBelongs to the DNA polymerase type-Y family.
Contains 1 umuC domain.
DomainThe catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.
Cellular localizationNucleus. Accumulates at replication forks after DNA damage.
- Information by UniProt
- DNA polymerase iota antibody
- Eta 2 antibody
- Eta2 antibody
All lanes : Anti-DNA Polymerase iota antibody (ab264193) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 30 seconds
Secondary: Goat anti-Rabbit Light Chain HRP Conjugate.
DNA Polymerase iota was immunoprecipitated from 1 mg HeLa whole cell lysate using ab264193 at 3 µg/mg lysate. Western blot was performed on the immunoprecipitate using ab264193 at 1 µg/ml.
Lane 1: ab264193 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264193 has not yet been referenced specifically in any publications.