Recombinant
RabMAb

Anti-DNA Polymerase lambda antibody [EPR7519(2)] (ab172974)

Overview

  • Product name
    Anti-DNA Polymerase lambda antibody [EPR7519(2)]
    See all DNA Polymerase lambda primary antibodies
  • Description
    Rabbit monoclonal [EPR7519(2)] to DNA Polymerase lambda
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DNA Polymerase lambda aa 550 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9UGP5

  • Positive control
    • HepG2, HeLa and 293T cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR7519(2)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab172974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 63 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function
      Repair polymerase. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Has both DNA polymerase and terminal transferase activities. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
    • Tissue specificity
      Expressed in a number of tissues. Abundant in testis.
    • Sequence similarities
      Belongs to the DNA polymerase type-X family.
      Contains 1 BRCT domain.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • BETA N antibody
      • BETAN antibody
      • DNA directed DNA polymerase lambda antibody
      • DNA polymerase beta 2 antibody
      • DNA polymerase beta-2 antibody
      • DNA polymerase kappa antibody
      • DNA polymerase kappa DNA polymerase beta N antibody
      • DNA polymerase lambda antibody
      • DNA polymerase lamda2 antibody
      • DPOLL_HUMAN antibody
      • EC 2.7.7.7,EC 4.2.99. antibody
      • FLJ46002 antibody
      • OTTHUMP00000020321 antibody
      • OTTHUMP00000020323 antibody
      • OTTHUMP00000059179 antibody
      • Pol beta2 antibody
      • POL KAPPA antibody
      • Pol Lambda antibody
      • POLKAPPA antibody
      • POLL antibody
      • Polymerase DNA directed lambda antibody
      see all

    Images

    • All lanes : Anti-DNA Polymerase lambda antibody [EPR7519(2)] (ab172974) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 63 kDa

    References

    This product has been referenced in:
    • Qi Y  et al. DNA Polymerase ? Is a Key Cellular Factor for the Formation of Covalently Closed Circular DNA of Hepatitis B Virus. PLoS Pathog 12:e1005893 (2016). Human . Read more (PubMed: 27783675) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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