Key features and details
- Rabbit polyclonal to DNA Polymerase lambda/Polk
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DNA Polymerase lambda/Polk antibody
See all DNA Polymerase lambda/Polk primary antibodies
DescriptionRabbit polyclonal to DNA Polymerase lambda/Polk
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
Synthetic peptide within Human DNA Polymerase lambda/Polk aa 150-200. The exact sequence is proprietary. (NP_037406.1).
Database link: Q9UGP5
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysates.
This product was previously labelled as DNA Polymerase lambda
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab224823 was affinity purified using an epitope specific to DNA Polymerase lambda/Polk immobilized on solid support.
Our Abpromise guarantee covers the use of ab224823 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 63 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionRepair polymerase. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Has both DNA polymerase and terminal transferase activities. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
Tissue specificityExpressed in a number of tissues. Abundant in testis.
Sequence similaritiesBelongs to the DNA polymerase type-X family.
Contains 1 BRCT domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- BETA N antibody
- BETAN antibody
- DNA directed DNA polymerase lambda antibody
All lanes : Anti-DNA Polymerase lambda/Polk antibody (ab224823) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 63 kDa
Exposure time: 3 minutes
Lystates prepared using NETN lysis buffer.
DNA Polymerase lambda/Polk was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN lysis buffer) with ab224823 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab224823 at 1 µg/ml.
Lane 1: ab224823 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224823 has not yet been referenced specifically in any publications.