Product nameAnti-DNA Polymerase lambda/Polk antibody
See all DNA Polymerase lambda/Polk primary antibodies
DescriptionRabbit polyclonal to DNA Polymerase lambda/Polk
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
Synthetic peptide within Human DNA Polymerase lambda/Polk aa 150-200. The exact sequence is proprietary. (NP_037406.1).
Database link: Q9UGP5
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysates.
This product was previously labelled as DNA Polymerase lambda
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab224823 was affinity purified using an epitope specific to DNA Polymerase lambda/Polk immobilized on solid support.
Our Abpromise guarantee covers the use of ab224823 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 63 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionRepair polymerase. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Has both DNA polymerase and terminal transferase activities. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
Tissue specificityExpressed in a number of tissues. Abundant in testis.
Sequence similaritiesBelongs to the DNA polymerase type-X family.
Contains 1 BRCT domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- BETA N antibody
- BETAN antibody
- DNA directed DNA polymerase lambda antibody
All lanes : Anti-DNA Polymerase lambda/Polk antibody (ab224823) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 63 kDa
Exposure time: 3 minutes
Lystates prepared using NETN lysis buffer.
DNA Polymerase lambda/Polk was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN lysis buffer) with ab224823 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab224823 at 1 µg/ml.
Lane 1: ab224823 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
ab224823 has not yet been referenced specifically in any publications.