Anti-DNA Polymerase lambda antibody (ab236985)
Key features and details
- Rabbit polyclonal to DNA Polymerase lambda
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-DNA Polymerase lambda antibody
See all DNA Polymerase lambda primary antibodies -
Description
Rabbit polyclonal to DNA Polymerase lambda -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment corresponding to Human DNA Polymerase lambda aa 1-250.
Database link: Q9UGP5 -
Positive control
- WB: Rat heart and lung tissue lysates. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236985 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/200 - 1/2000. Predicted molecular weight: 63 kDa.
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ICC/IF |
1/50 - 1/200.
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Notes |
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WB
1/200 - 1/2000. Predicted molecular weight: 63 kDa. |
ICC/IF
1/50 - 1/200. |
Target
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Function
Repair polymerase. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Has both DNA polymerase and terminal transferase activities. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity. -
Tissue specificity
Expressed in a number of tissues. Abundant in testis. -
Sequence similarities
Belongs to the DNA polymerase type-X family.
Contains 1 BRCT domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 27343 Human
- Entrez Gene: 361767 Rat
- Omim: 606343 Human
- SwissProt: Q9UGP5 Human
- SwissProt: Q5RKI3 Rat
- Unigene: 523230 Human
- Unigene: 11799 Rat
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Alternative names
- BETA N antibody
- BETAN antibody
- DNA directed DNA polymerase lambda antibody
see all
Images
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All lanes : Anti-DNA Polymerase lambda antibody (ab236985) at 1/200 dilution
Lane 1 : Rat lung tissue lysate
Lane 2 : Rat heart tissue lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 63 kDa -
HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for DNA Polymerase lambda (green) using ab236985 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab236985 has not yet been referenced specifically in any publications.