Key features and details
- Rabbit polyclonal to DNA Polymerase lambda/Polk
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-DNA Polymerase lambda/Polk antibody
See all DNA Polymerase lambda/Polk primary antibodies
DescriptionRabbit polyclonal to DNA Polymerase lambda/Polk
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Recombinant fragment corresponding to Human DNA Polymerase lambda/Polk aa 16-211.
Database link: Q9UGP5
- WB: Rat heart and lung tissue lysates. ICC/IF: HeLa cells.
This product was previously labelled as DNA Polymerase lambda
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236985 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/200 - 1/2000. Predicted molecular weight: 63 kDa.|
|ICC/IF||1/50 - 1/200.|
FunctionRepair polymerase. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Has both DNA polymerase and terminal transferase activities. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
Tissue specificityExpressed in a number of tissues. Abundant in testis.
Sequence similaritiesBelongs to the DNA polymerase type-X family.
Contains 1 BRCT domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- BETA N antibody
- BETAN antibody
- DNA directed DNA polymerase lambda antibody
All lanes : Anti-DNA Polymerase lambda/Polk antibody (ab236985) at 1/200 dilution
Lane 1 : Rat lung tissue lysate
Lane 2 : Rat heart tissue lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 63 kDa
HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for DNA Polymerase lambda/Polk (green) using ab236985 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236985 has not yet been referenced specifically in any publications.