Overview

  • Product name

    Anti-DNA Polymerase lambda/Polk antibody
    See all DNA Polymerase lambda/Polk primary antibodies
  • Description

    Rabbit polyclonal to DNA Polymerase lambda/Polk
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human DNA Polymerase lambda/Polk aa 16-211.
    Database link: Q9UGP5

  • Positive control

    • WB: Rat heart and lung tissue lysates. ICC/IF: HeLa cells.
  • General notes

     This product was previously labelled as DNA Polymerase lambda

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab236985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/2000. Predicted molecular weight: 63 kDa.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Repair polymerase. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Has both DNA polymerase and terminal transferase activities. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
  • Tissue specificity

    Expressed in a number of tissues. Abundant in testis.
  • Sequence similarities

    Belongs to the DNA polymerase type-X family.
    Contains 1 BRCT domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • BETA N antibody
    • BETAN antibody
    • DNA directed DNA polymerase lambda antibody
    • DNA polymerase beta 2 antibody
    • DNA polymerase beta-2 antibody
    • DNA polymerase kappa antibody
    • DNA polymerase kappa DNA polymerase beta N antibody
    • DNA polymerase lambda antibody
    • DNA polymerase lamda2 antibody
    • DPOLL_HUMAN antibody
    • EC 2.7.7.7,EC 4.2.99. antibody
    • FLJ46002 antibody
    • OTTHUMP00000020321 antibody
    • OTTHUMP00000020323 antibody
    • OTTHUMP00000059179 antibody
    • Pol beta2 antibody
    • POL KAPPA antibody
    • Pol Lambda antibody
    • POLKAPPA antibody
    • POLL antibody
    • Polymerase DNA directed lambda antibody
    see all

Images

  • All lanes : Anti-DNA Polymerase lambda/Polk antibody (ab236985) at 1/200 dilution

    Lane 1 : Rat lung tissue lysate
    Lane 2 : Rat heart tissue lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 63 kDa

  • HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for DNA Polymerase lambda/Polk (green) using ab236985 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

References

ab236985 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab236985.
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