Key features and details
- Rabbit polyclonal to DNA polymerase mu
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DNA polymerase mu antibody
See all DNA polymerase mu primary antibodies
DescriptionRabbit polyclonal to DNA polymerase mu
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
- ICC/IF: U-251 MG cells. WB: RT4 cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab221653 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 55 kDa.|
FunctionGap-filling polymerase involved in repair of DNA double-strand breaks by non-homologous end joining (NHEJ). Participates in immunoglobulin (Ig) light chain gene rearrangement in V(D)J recombination.
Tissue specificityExpressed in a number of tissues. Abundant in thymus.
Sequence similaritiesBelongs to the DNA polymerase type-X family.
Contains 1 BRCT domain.
- Information by UniProt
- DNA-directed DNA polymerase mu antibody
- DNA-directed DNA/RNA polymerase mu antibody
- DPOLM_HUMAN antibody
PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for DNA polymerase mu (green) using ab221653 at 4 µg/ml in ICC/IF.
Anti-DNA polymerase mu antibody (ab221653) at 1/250 dilution + RT4 (human urinary bladder cancer cell line) cell lysate
Predicted band size: 55 kDa
ab221653 has not yet been referenced specifically in any publications.