Overview

  • Product name

    Anti-DNA polymerase mu antibody [EPR10470(B)]
    See all DNA polymerase mu primary antibodies
  • Description

    Rabbit monoclonal [EPR10470(B)] to DNA polymerase mu
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human DNA polymerase mu (UniProt Q9NP87).

  • Positive control

    • 293T, HeLa and Jurkat cell lysates; Immunoprecipitation pellet from Jurkat cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR10470(B)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab157465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 55 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function

      Gap-filling polymerase involved in repair of DNA double-strand breaks by non-homologous end joining (NHEJ). Participates in immunoglobulin (Ig) light chain gene rearrangement in V(D)J recombination.
    • Tissue specificity

      Expressed in a number of tissues. Abundant in thymus.
    • Sequence similarities

      Belongs to the DNA polymerase type-X family.
      Contains 1 BRCT domain.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • DNA-directed DNA polymerase mu antibody
      • DNA-directed DNA/RNA polymerase mu antibody
      • DPOLM_HUMAN antibody
      • FLJ35482 antibody
      • Pol iota antibody
      • Pol Mu antibody
      • Polm antibody
      • POLM protein antibody
      • Polymerase (DNA directed) mu antibody
      • Polymerase DNA directed mu antibody
      • Tdt N antibody
      • Tdt-N antibody
      • TdtN antibody
      • Terminal transferase antibody
      see all

    Images

    • All lanes : Anti-DNA polymerase mu antibody [EPR10470(B)] (ab157465) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 55 kDa

    • Anti-DNA polymerase mu antibody [EPR10470(B)] (ab157465) at 1/1000 dilution + immunoprecipitation pellet from Jurkat cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 55 kDa

    References

    This product has been referenced in:

    • Schimmel J  et al. Mutational signatures of non-homologous and polymerase theta-mediated end-joining in embryonic stem cells. EMBO J N/A:N/A (2017). WB ; Mouse . Read more (PubMed: 29079701) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab157465.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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