Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10470(B)] to DNA polymerase mu - BSA and Azide free
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Product nameAnti-DNA polymerase mu antibody [EPR10470(B)] - BSA and Azide free
See all DNA polymerase mu primary antibodies
DescriptionRabbit monoclonal [EPR10470(B)] to DNA polymerase mu - BSA and Azide free
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human DNA polymerase mu. The exact sequence is proprietary.
Database link: Q9NP87
- WB: HeLa, HEK-293T and Daudi cell lysates.
Ab249333 is the carrier-free version of ab157465. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab249333 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
KO cell lysates
Our Abpromise guarantee covers the use of ab249333 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 55 kDa.|
|IP||Use at an assay dependent concentration.|
FunctionGap-filling polymerase involved in repair of DNA double-strand breaks by non-homologous end joining (NHEJ). Participates in immunoglobulin (Ig) light chain gene rearrangement in V(D)J recombination.
Tissue specificityExpressed in a number of tissues. Abundant in thymus.
Sequence similaritiesBelongs to the DNA polymerase type-X family.
Contains 1 BRCT domain.
- Information by UniProt
- DNA-directed DNA polymerase mu antibody
- DNA-directed DNA/RNA polymerase mu antibody
- DPOLM_HUMAN antibody
All lanes : Anti-DNA polymerase mu antibody [EPR10470(B)] (ab157465) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : DNA polymerase mu knockout HeLa cell lysate
Lane 3 : HEK-293T cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 55 kDa
This data was developed using the same antibody clone in a different buffer formulation (ab157465).
ab157465 Anti-DNA polymerase mu antibody [EPR10470(B)] was shown to specifically react with POLM in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265557 (knockout cell lysate ab258127) was used. Wild-type and POLM knockout samples were subjected to SDS-PAGE. ab157465 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab249333 has not yet been referenced specifically in any publications.