Overview

  • Product name

    DNA-Protein Binding Assay Kit (Colorimetric)
    See all DNA-protein kits
  • Detection method

    Colorimetric
  • Assay time

    3h 00m
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Mammals
  • Product overview

    Protein-DNA interactions play a critical role for cellular functions such as signal transduction, gene transcription and epigenetic silencing. Identifying the genetic targets of DNA binding proteins and knowing the mechanishm of protein-DNA interaction is important for understanding cellular processes.

    DNA-Protein Binding Assay Kit (Colorimetric) (ab117139) allows the user to investigate direct in vitro protein-DNA interaction, using dsDNA of choice, within 3 hours. ab117139 is specifically suitable for detection of transcription factor activation in mammalian tissue and cell extracts.

  • Platform

    Microplate reader

Properties

Protocols

References

This product has been referenced in:

  • Kwon HK  et al. FoxP3 scanning mutagenesis reveals functional variegation and mild mutations with atypical autoimmune phenotypes. Proc Natl Acad Sci U S A 115:E253-E262 (2018). Read more (PubMed: 29269391) »
  • Kwon HK  et al. Different molecular complexes that mediate transcriptional induction and repression by FoxP3. Nat Immunol 18:1238-1248 (2017). Read more (PubMed: 28892470) »
See all 3 Publications for this product

Customer reviews and Q&As

Answer

Thank you for contacting us and your interest in our products.



The EpiSeeker DNA-Protein Binding Assay Kit (Colorimetric) ab117139 should be suitable for the experiment you are planning with your plant tissues. This kit provides the core components for the DNA-protein binding assay. You will need to prepare your own biotin-ds Oligos, primary antibody, secondary antibody as well as nuclear extracts from your plant samples. The assay can then be performed as per the protocol instructions provided with the kit.



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