Product nameDNA-Protein Binding Assay Kit (Colorimetric)
See all DNA-protein kits
Assay time3h 00m
Species reactivityReacts with: Mouse, Human
Predicted to work with: Mammals
Protein-DNA interactions play a critical role for cellular functions such as signal transduction, gene transcription and epigenetic silencing. Identifying the genetic targets of DNA binding proteins and knowing the mechanishm of protein-DNA interaction is important for understanding cellular processes.
DNA-Protein Binding Assay Kit (Colorimetric) (ab117139) allows the user to investigate direct in vitro protein-DNA interaction, using dsDNA of choice, within 3 hours. ab117139 is specifically suitable for detection of transcription factor activation in mammalian tissue and cell extracts.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 96 tests 10X Wash Buffer 1 x 20ml 8 Well Assay Strips (with Frame) 12 units Antibody Dilution Buffer 1 x 20ml Assay Binding Buffer 1 x 4ml Developing Solution 1 x 10ml Stop Solution 1 x 6ml
ab117139 has been referenced in 4 publications.
- Blanco-Cantó ME et al. Novel EGR2 variant that associates with Charcot-Marie-Tooth disease when combined with lipopolysaccharide-induced TNF-a factor T49M polymorphism. Neurol Genet 6:e407 (2020). PubMed: 32337334
- Kwon HK et al. FoxP3 scanning mutagenesis reveals functional variegation and mild mutations with atypical autoimmune phenotypes. Proc Natl Acad Sci U S A 115:E253-E262 (2018). PubMed: 29269391
- Kwon HK et al. Different molecular complexes that mediate transcriptional induction and repression by FoxP3. Nat Immunol 18:1238-1248 (2017). PubMed: 28892470
- Lee YL et al. MAF1 represses CDKN1A through a Pol III-dependent mechanism. Elife 4:e06283 (2015). Functional Studies . PubMed: 26067234