Anti-DNA2 antibody (ab96488)
Key features and details
- Rabbit polyclonal to DNA2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DNA2 antibody
See all DNA2 primary antibodies -
Description
Rabbit polyclonal to DNA2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to Human DNA2 aa 1000 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab42548) -
Positive control
- This antibody gave a positive signal in the following Human Whole Cell lysates; HEK293, HEK293 (Nuclear) and U2OS and FFPE human liver tissue sections.
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General notes
ab96488 is raised using the same immunogen as ab42439. Batches sold under ab96488 have passed QC testing in Western Blot. In our hands, this product did not work in ICC/IF. For further information please contact our Customer Services team.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab96488 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 120 kDa). | |
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
May function in chromosomal DNA replication. -
Sequence similarities
Belongs to the DNA2/NAM7 helicase family. - Information by UniProt
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Database links
- Entrez Gene: 1763 Human
- Entrez Gene: 327762 Mouse
- Entrez Gene: 309762 Rat
- Omim: 601810 Human
- SwissProt: P51530 Human
- SwissProt: Q6ZQJ5 Mouse
- SwissProt: D3ZG52 Rat
- Unigene: 532446 Human
see all -
Alternative names
- DNA replication ATP-dependent helicase-like homolog antibody
- DNA replication helicase 2 homolog antibody
- DNA replication helicase 2, yeast, homolog of antibody
see all
Images
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Western blot - Anti-DNA2 antibody (ab96488)All lanes : Anti-DNA2 antibody (ab96488) at 1 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Nuclear Lysate
Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 120 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
Additional bands at: 16 kDa, 18 kDa, 210 kDa, 37 kDa, 38 kDa, 54 kDa, 60 kDa, 70 kDa, 76 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
This antibody detects multiple bands in addition to the band of interest, reflecting low levels of expression of DNA2. siRNA knockdown experiments have demonstrated that this antibody detects a band at ~120 kDa corresponding to human DNA2 (please see image courtesy of Julien Duxin). -
Western blot - Anti-DNA2 antibody (ab96488)Image courtesy of Julien Duxin and Sheila StewartAll lanes : Anti-DNA2 antibody (ab96488)
Lane 1 : U2OS cells plus control shRNA
Lane 2 : U2OS cells plus shDNA2-1 shRNA
Lane 3 : U2OS cells plus shDNA2-2 shRNA
Secondary
All lanes : Anti-rabbit at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 120 kDa
Observed band size: 120 kDa
Western blot showing detection of DNA2 by ab42439. A 120 kDa band corresponding to DNA2 was detected in lysates of U2OS cells treated with control shRNA (lane 1) but not in those from knockdown cells treated with two different shRNAs for DNA2 (lanes 2 and 3). Following pre-blocking for 30 mins in 5% milk, PBS-Tween, the membrane was incubated overnight at 4°C with ab42439 at a dilution of 1/1000 in 5% milk, PBS-Tween. The membrane was then incubated with the secondary antibody at 1/3000 dilution in 5% milk, PBS-Tween for 2 hours at RT, and washed 3 x 10 mins with PBS-Tween prior to ECL detection. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA2 antibody (ab96488)IHC image of ab96488 staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab96488, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
References (22)
ab96488 has been referenced in 22 publications.
- Yu H et al. Global crotonylome reveals CDYL-regulated RPA1 crotonylation in homologous recombination-mediated DNA repair. Sci Adv 6:eaay4697 (2020). PubMed: 32201722
- Mariotti L et al. The iron-sulphur cluster in human DNA2 is required for all biochemical activities of DNA2. Commun Biol 3:322 (2020). PubMed: 32576938
- Garzón J et al. Human RIF1-Protein Phosphatase 1 Prevents Degradation and Breakage of Nascent DNA on Replication Stalling. Cell Rep 27:2558-2566.e4 (2019). PubMed: 31141682
- Mason JM et al. Non-enzymatic roles of human RAD51 at stalled replication forks. Nat Commun 10:4410 (2019). PubMed: 31562309
- Panier S et al. SLX4IP Antagonizes Promiscuous BLM Activity during ALT Maintenance. Mol Cell 76:27-43.e11 (2019). PubMed: 31447390
