Anti-DNA2 antibody (ab96488)
- Datasheet
- References (17)
- Protocols
Overview
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Product name
Anti-DNA2 antibody
See all DNA2 primary antibodies -
Description
Rabbit polyclonal to DNA2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to Human DNA2 aa 1000 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab42548) -
Positive control
- This antibody gave a positive signal in the following Human Whole Cell lysates; HEK293, HEK293 (Nuclear) and U2OS and FFPE human liver tissue sections.
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General notes
ab96488 is raised using the same immunogen as ab42439. Batches sold under ab96488 have passed QC testing in Western Blot. In our hands, this product did not work in ICC/IF. For further information please contact our Customer Services team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab96488 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 120 kDa). | |
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
May function in chromosomal DNA replication. -
Sequence similarities
Belongs to the DNA2/NAM7 helicase family. - Information by UniProt
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Database links
- Entrez Gene: 1763 Human
- Entrez Gene: 327762 Mouse
- Entrez Gene: 309762 Rat
- Omim: 601810 Human
- SwissProt: P51530 Human
- SwissProt: Q6ZQJ5 Mouse
- SwissProt: D3ZG52 Rat
- Unigene: 532446 Human
see all -
Alternative names
- DNA replication ATP-dependent helicase-like homolog antibody
- DNA replication helicase 2 homolog antibody
- DNA replication helicase 2, yeast, homolog of antibody
see all
Images
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Western blot - Anti-DNA2 antibody (ab96488)All lanes : Anti-DNA2 antibody (ab96488) at 1 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Nuclear Lysate
Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 120 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
Additional bands at: 16 kDa, 18 kDa, 210 kDa, 37 kDa, 38 kDa, 54 kDa, 60 kDa, 70 kDa, 76 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
This antibody detects multiple bands in addition to the band of interest, reflecting low levels of expression of DNA2. siRNA knockdown experiments have demonstrated that this antibody detects a band at ~120 kDa corresponding to human DNA2 (please see image courtesy of Julien Duxin). -
Western blot - Anti-DNA2 antibody (ab96488)Image courtesy of Julien Duxin and Sheila StewartAll lanes : Anti-DNA2 antibody (ab96488)
Lane 1 : U2OS cells plus control shRNA
Lane 2 : U2OS cells plus shDNA2-1 shRNA
Lane 3 : U2OS cells plus shDNA2-2 shRNA
Secondary
All lanes : Anti-rabbit at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 120 kDa
Observed band size: 120 kDa
Western blot showing detection of DNA2 by ab42439. A 120 kDa band corresponding to DNA2 was detected in lysates of U2OS cells treated with control shRNA (lane 1) but not in those from knockdown cells treated with two different shRNAs for DNA2 (lanes 2 and 3). Following pre-blocking for 30 mins in 5% milk, PBS-Tween, the membrane was incubated overnight at 4°C with ab42439 at a dilution of 1/1000 in 5% milk, PBS-Tween. The membrane was then incubated with the secondary antibody at 1/3000 dilution in 5% milk, PBS-Tween for 2 hours at RT, and washed 3 x 10 mins with PBS-Tween prior to ECL detection. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA2 antibody (ab96488)IHC image of ab96488 staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab96488, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Datasheets and documents
References
This product has been referenced in:
- Garzón J et al. Human RIF1-Protein Phosphatase 1 Prevents Degradation and Breakage of Nascent DNA on Replication Stalling. Cell Rep 27:2558-2566.e4 (2019). Read more (PubMed: 31141682) »
- Li Z et al. hDNA2 nuclease/helicase promotes centromeric DNA replication and genome stability. EMBO J 37:N/A (2018). Read more (PubMed: 29773570) »
