Anti-DNAH6 antibody (ab122333)
Key features and details
- Rabbit polyclonal to DNAH6
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DNAH6 antibody -
Description
Rabbit polyclonal to DNAH6 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
TKLNTILCQT FVFCYLWSLG GNLTENYYDS FDTFIRTQFD DNPDARLPNS GDLWSIHMDF DTKRLDPWER IIPTFKYNRD VP internal sequence amino acids 1983-2064 of Human DNAH6 (Q9C0G6).
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Positive control
- Human fallopian tube tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab122333 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
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| IHC-P | 1/850. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. -
Tissue specificity
Detected in brain, testis and trachea. -
Sequence similarities
Belongs to the dynein heavy chain family. -
Domain
Dynein heavy chains probably consist of an N-terminal stem (which binds cargo and interacts with other dynein components), and the head or motor domain. The motor contains six tandemly-linked AAA domains in the head, which form a ring. A stalk-like structure (formed by two of the coiled coil domains) protrudes between AAA 4 and AAA 5 and terminates in a microtubule-binding site. A seventh domain may also contribute to this ring; it is not clear whether the N-terminus or the C-terminus forms this extra domain. There are four well-conserved and two non-conserved ATPase sites, one per AAA domain. Probably only one of these (within AAA 1) actually hydrolyzes ATP, the others may serve a regulatory function. -
Cellular localization
Cytoplasm > cytoskeleton > cilium axoneme. - Information by UniProt
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Database links
- Entrez Gene: 1768 Human
- Omim: 603336 Human
- SwissProt: Q9C0G6 Human
- Unigene: 249972 Human
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Alternative names
- axonemal antibody
- Axonemal beta dynein heavy chain 6 antibody
- Ciliary dynein heavy chain 6 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-DNAH6 antibody (ab122333)Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli, plasma membrane and cytoplasm. Recommended concentration of ab122333 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAH6 antibody (ab122333)ab122333, at a 1/850 dilution, staining DNAH6 in glandular cells of paraffin-embedded Human fallopian tube tissue by immunohistochemistry.
Protocols
Datasheets and documents
References (1)
ab122333 has been referenced in 1 publication.
- Tu C et al. Identification of DNAH6 mutations in infertile men with multiple morphological abnormalities of the sperm flagella. Sci Rep 9:15864 (2019). PubMed: 31676830
