Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11224] to DNAI2
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Rat, Human
Product nameAnti-DNAI2 antibody [EPR11224]
See all DNAI2 primary antibodies
DescriptionRabbit monoclonal [EPR11224] to DNAI2
Tested applicationsSuitable for: WB, IHC-P, IPmore details
Unsuitable for: ICC/IF
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human DNAI2. The exact sequence is proprietary.
Database link: Q9GZS0
- Human testis, Rat testis, Mouse testis and fetal lung lysates.
This product was previously labelled as Dynein intermediate chain 2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab170920 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 69 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||1/10 - 1/100.|
FunctionPart of the dynein complex of respiratory cilia.
Tissue specificityHighly expressed in trachea and testis.
Involvement in diseaseDefects in DNAI2 are the cause of primary ciliary dyskinesia type 9 (CILD9) [MIM:612444]. CILD is an autosomal recessive disorder characterized by axonemal abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit situs inversus, due to dysfunction of monocilia at the embryonic node and randomization of left-right body asymmetry. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome.
Sequence similaritiesBelongs to the dynein intermediate chain family.
Contains 5 WD repeats.
Cellular localizationCytoplasm > cytoskeleton > cilium axoneme.
- Information by UniProt
- axonemal antibody
- Axonemal dynein intermediate chain 2 antibody
- CILD9 antibody
Immunohistochemical analysis of paraffin embedded testis tissue labeling DNAI2 with ab170920 at 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-DNAI2 antibody [EPR11224] (ab170920) at 1/1000 dilution + Immunoprecipitation pellet from human testis lysate using ab170920 at 1/10.
Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Developed using the ECL technique.
Additional bands at: ~50 kDa (possible IgG)
All lanes : Anti-DNAI2 antibody [EPR11224] (ab170920) at 1/1000 dilution
Lane 1 : Human testis lysate
Lane 2 : Rat testis lysate
Lane 3 : Mouse testis lysate
Lane 4 : Fetal lung lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 69 kDa
ab170920 has not yet been referenced specifically in any publications.