Recombinant Anti-DNAI2 antibody [EPR11224] (ab170920)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11224] to DNAI2
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-DNAI2 antibody [EPR11224]
See all DNAI2 primary antibodies -
Description
Rabbit monoclonal [EPR11224] to DNAI2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human DNAI2. The exact sequence is proprietary.
Database link: Q9GZS0 -
Positive control
- Human testis, Rat testis, Mouse testis and fetal lung lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11224 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab170920 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 69 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
1/10 - 1/100.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 69 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
1/10 - 1/100. |
Target
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Function
Part of the dynein complex of respiratory cilia. -
Tissue specificity
Highly expressed in trachea and testis. -
Involvement in disease
Defects in DNAI2 are the cause of primary ciliary dyskinesia type 9 (CILD9) [MIM:612444]. CILD is an autosomal recessive disorder characterized by axonemal abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit situs inversus, due to dysfunction of monocilia at the embryonic node and randomization of left-right body asymmetry. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome. -
Sequence similarities
Belongs to the dynein intermediate chain family.
Contains 5 WD repeats. -
Cellular localization
Cytoplasm > cytoskeleton > cilium axoneme. - Information by UniProt
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Database links
- Entrez Gene: 64446 Human
- Entrez Gene: 432611 Mouse
- Entrez Gene: 360654 Rat
- Omim: 605483 Human
- SwissProt: Q9GZS0 Human
- SwissProt: A2AC93 Mouse
- SwissProt: Q66HC9 Rat
- Unigene: 147472 Human
see all -
Alternative names
- axonemal antibody
- Axonemal dynein intermediate chain 2 antibody
- CILD9 antibody
see all
Images
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Immunohistochemical analysis of paraffin embedded testis tissue labeling DNAI2 with ab170920 at 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Anti-DNAI2 antibody [EPR11224] (ab170920) at 1/1000 dilution + Immunoprecipitation pellet from human testis lysate using ab170920 at 1/10.
Secondary
Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Developed using the ECL technique.
Additional bands at: ~50 kDa (possible IgG) -
All lanes : Anti-DNAI2 antibody [EPR11224] (ab170920) at 1/1000 dilution
Lane 1 : Human testis lysate
Lane 2 : Rat testis lysate
Lane 3 : Mouse testis lysate
Lane 4 : Fetal lung lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 69 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab170920 has not yet been referenced specifically in any publications.