Key features and details
- Rabbit polyclonal to DNAJA1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DNAJA1 antibody
See all DNAJA1 primary antibodies
DescriptionRabbit polyclonal to DNAJA1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, African green monkey, Orangutan
- WB: HeLa whole cell lysate. IHC-P: Human placenta tissue. ICC/IF: HepG2 cells.
This product was previously labelled as HDJ2
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab234702 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).|
|IHC-P||1/1000 - 1/2000.|
|ICC/IF||1/50 - 1/500.|
FunctionCo-chaperone of Hsc70. Seems to play a role in protein import into mitochondria.
Sequence similaritiesContains 1 CR-type zinc finger.
Contains 1 J domain.
- Information by UniProt
- DJ 2 antibody
- DJ2 antibody
- DjA1 antibody
Anti-DNAJA1 antibody (ab234702) at 1/500 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
Paraffin-embedded human placenta tissue stained for DNAJA1 using ab234702 at 1/1000 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for DNAJA1 (green) using ab234702 at 1/100 dilution in ICC/IF. Secondary antibody is Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG(H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab234702 has not yet been referenced specifically in any publications.