Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-DNAJA1 antibody [EPR7248] (ab126774)

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Overview

  • Product name

    Anti-DNAJA1 antibody [EPR7248]
    See all DNAJA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR7248] to DNAJA1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human DNAJA1 aa 350-450 (C terminal). The exact sequence is proprietary.

  • Positive control

    • HepG2, SKBR 3, Jurkat and Raji cell lysates; Jurkat cells.

  • General notes

     

     

     This product was previously labelled as HDJ2

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 4.20 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR7248

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 46 kDa (predicted molecular weight: 45 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.

    • Target

    • Function

      Co-chaperone of Hsc70. Seems to play a role in protein import into mitochondria.

    • Sequence similarities

      Contains 1 CR-type zinc finger.
      Contains 1 J domain.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • DJ 2 antibody
      • DJ2 antibody
      • DjA1 antibody
      • DnaJ (Hsp40) homolog, subfamily A, member 1 antibody
      • DnaJ homolog subfamily A member 1 antibody
      • DnaJ protein homolog 2 antibody
      • DNAJ2 antibody
      • Dnaja1 antibody
      • DNJA1_HUMAN antibody
      • hDJ 2 antibody
      • HDJ-2 antibody
      • HDJ2 antibody
      • Heat shock 40 kDa protein 4 antibody
      • heat shock protein DNAJ like 2 antibody
      • Heat shock protein J2 antibody
      • HSDJ antibody
      • HSJ-2 antibody
      • HSJ2 antibody
      • HSPF4 antibody
      • Human DnaJ protein 2 antibody
      • NEDD7 antibody
      • Neural precursor cell expressed developmentally down regulated 7 antibody
      • OTTHUMP00000021193 antibody
      see all

    Images

    • Western blot - Anti-DNAJA1 antibody [EPR7248] (ab126774)
      Western blot - Anti-DNAJA1 antibody [EPR7248] (ab126774)

      Lane 1: Wild-type DNAJA1 whole cell lysate (20 µg)              
      Lane 2: DNAJA1 knockout  DNAJA1 whole cell lysate (20 µg)
      Lane 3: HepG2 whole cell lysate (20 µg)
      Lane 4: Jurkat whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab126774 observed at 45 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab126774 was shown to recognize DNAJA1 in wild-type HAP1 samples along with additional cross-reactive bands. No band was observed when DNAJA1 knockout samples were examined. Wild-type and DNAJA1 knockout samples were subjected to SDS-PAGE.  Ab126774 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/10,000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    • Flow Cytometry - Anti-DNAJA1 antibody [EPR7248] (ab126774)
      Flow Cytometry - Anti-DNAJA1 antibody [EPR7248] (ab126774)

      ab126774 at 1/10 dilution staining DNAJA1 in permeabilized Jurkat cells by Flow cytometry (red). Rabbit IgG negative control (green).

    • Western blot - Anti-DNAJA1 antibody [EPR7248] (ab126774)
      Western blot - Anti-DNAJA1 antibody [EPR7248] (ab126774)
      All lanes : Anti-DNAJA1 antibody [EPR7248] (ab126774) at 1/10000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : SKBR 3 cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 45 kDa

    • Other - Anti-DNAJA1 antibody [EPR7248] (ab126774)
      Other - Anti-DNAJA1 antibody [EPR7248] (ab126774)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Wang C  et al. Heat shock protein DNAJA1 stabilizes PIWI proteins to support regeneration and homeostasis of planarian Schmidtea mediterranea. J Biol Chem 294:9873-9887 (2019). Read more (PubMed: 31076507) »
    See 1 Publication for this product

    Publishing research using ab126774? Please let us know so that we can cite the reference in this datasheet.

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