Recombinant Anti-DNAJA1 antibody [EPR7248] (ab126774)
![Western blot - Anti-DNAJA1 antibody [EPR7248] (ab126774)](https://www.abcam.com/ps/products/126/ab126774/Images/ab126774-393618-anti-dnaja1-antibody-epr7248-western-blot-human-knockout-lysate.jpg "Western blot - Anti-DNAJA1 antibody [EPR7248] (ab126774)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7248] to DNAJA1
- Suitable for: Flow Cyt (Intra), WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-DNAJA1 antibody [EPR7248]
See all DNAJA1 primary antibodies -
Description
Rabbit monoclonal [EPR7248] to DNAJA1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human DNAJA1 aa 350-450 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: HEK293T, Jurkat, HepG2, SKBR 3, Jurkat and Raji cell lysates. Flow Cyt (intra): Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.20 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7248 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126774 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/10000 - 1/50000. Detects a band of approximately 46 kDa (predicted molecular weight: 45 kDa).
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| Notes |
|---|
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/10000 - 1/50000. Detects a band of approximately 46 kDa (predicted molecular weight: 45 kDa). |
Target
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Function
Co-chaperone of Hsc70. Seems to play a role in protein import into mitochondria. -
Sequence similarities
Contains 1 CR-type zinc finger.
Contains 1 J domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3301 Human
- Entrez Gene: 15502 Mouse
- Entrez Gene: 65028 Rat
- Omim: 602837 Human
- SwissProt: P31689 Human
- SwissProt: P63037 Mouse
- SwissProt: P63036 Rat
- Unigene: 445203 Human
see all -
Alternative names
- DJ 2 antibody
- DJ2 antibody
- DjA1 antibody
see all
Images
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Western blot - Anti-DNAJA1 antibody [EPR7248] (ab126774)All lanes : Anti-DNAJA1 antibody [EPR7248] (ab126774) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : DNAJA1 knockout HEK293T cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 45 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab126774 observed at 49 kDa. Red - loading control ab8245 observed at 36 kDa.
ab126774 Anti-DNAJA1 antibody [EPR7248] was shown to specifically react with DNAJA1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266437 (knockout cell lysate ab257925) was used. Wild-type and DNAJA1 knockout samples were subjected to SDS-PAGE. ab126774 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-DNAJA1 antibody [EPR7248] (ab126774)](https://www.abcam.com/ps/products/126/ab126774/Images/ab126774-279870-anti-hdj2-antibody-epr7248-western-blot.jpg)
Western blot - Anti-DNAJA1 antibody [EPR7248] (ab126774)Lane 1: Wild-type DNAJA1 whole cell lysate (20 µg)
Lane 2: DNAJA1 knockout DNAJA1 whole cell lysate (20 µg)
Lane 3: HepG2 whole cell lysate (20 µg)
Lane 4: Jurkat whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab126774 observed at 45 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab126774 was shown to recognize DNAJA1 in wild-type HAP1 samples along with additional cross-reactive bands. No band was observed when DNAJA1 knockout samples were examined. Wild-type and DNAJA1 knockout samples were subjected to SDS-PAGE. Ab126774 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/10,000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Flow Cytometry (Intracellular) - Anti-DNAJA1 antibody [EPR7248] (ab126774)](https://www.abcam.com/ps/products/126/ab126774/Images/ab126774-140827-anti-hdj2-antibody-epr7248-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-DNAJA1 antibody [EPR7248] (ab126774)ab126774 at 1/10 dilution staining DNAJA1 in permeabilized Jurkat cells by intracellular flow cytometry (red). Rabbit IgG negative control (green).
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![Western blot - Anti-DNAJA1 antibody [EPR7248] (ab126774)](https://www.abcam.com/ps/products/126/ab126774/Images/ab126774-140828-anti-hdj2-antibody-epr7248-western-blot.jpg)
Western blot - Anti-DNAJA1 antibody [EPR7248] (ab126774)All lanes : Anti-DNAJA1 antibody [EPR7248] (ab126774) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : SKBR 3 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 45 kDa -
![OI-RD Scanning - Anti-DNAJA1 antibody [EPR7248] (ab126774)](https://www.abcam.com/ps/products/126/ab126774/Images/ab126774-140826-anti-hdj2-antibody-epr7248.jpg)
OI-RD Scanning - Anti-DNAJA1 antibody [EPR7248] (ab126774) -
![Anti-DNAJA1 antibody [EPR7248] (ab126774)](https://www.abcam.com/ps/products/126/ab126774/Images/ab126774-6-benefits-of-recombinant-antibodies.png)
Anti-DNAJA1 antibody [EPR7248] (ab126774)
Datasheets and documents
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Datasheet download
References (3)
ab126774 has been referenced in 3 publications.
- Egawa N et al. Antitumor effects of low-dose tipifarnib on the mTOR signaling pathway and reactive oxygen species production in HIF-1a-expressing gastric cancer cells. FEBS Open Bio 11:1465-1475 (2021). PubMed: 33773069
- Wang C et al. Heat shock protein DNAJA1 stabilizes PIWI proteins to support regeneration and homeostasis of planarian Schmidtea mediterranea. J Biol Chem 294:9873-9887 (2019). PubMed: 31076507
- Fiore D et al. N6-Isopentenyladenosine Inhibits Colorectal Cancer and Improves Sensitivity to 5-Fluorouracil-Targeting FBXW7 Tumor Suppressor. Cancers (Basel) 11:N/A (2019). PubMed: 31569395
