Product nameAnti-DNAJA1 antibody [EPR7248]
See all DNAJA1 primary antibodies
DescriptionRabbit monoclonal [EPR7248] to DNAJA1
Tested applicationsSuitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human DNAJA1 aa 350-450 (C terminal). The exact sequence is proprietary.
- HepG2, SKBR 3, Jurkat and Raji cell lysates; Jurkat cells.
This product was previously labelled as HDJ2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 4.20 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab126774 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 46 kDa (predicted molecular weight: 45 kDa).|
|IP||1/10 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionCo-chaperone of Hsc70. Seems to play a role in protein import into mitochondria.
Sequence similaritiesContains 1 CR-type zinc finger.
Contains 1 J domain.
- Information by UniProt
- DJ 2 antibody
- DJ2 antibody
- DjA1 antibody
Lane 1: Wild-type DNAJA1 whole cell lysate (20 µg)
Lane 2: DNAJA1 knockout DNAJA1 whole cell lysate (20 µg)
Lane 3: HepG2 whole cell lysate (20 µg)
Lane 4: Jurkat whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab126774 observed at 45 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab126774 was shown to recognize DNAJA1 in wild-type HAP1 samples along with additional cross-reactive bands. No band was observed when DNAJA1 knockout samples were examined. Wild-type and DNAJA1 knockout samples were subjected to SDS-PAGE. Ab126774 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/10,000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
ab126774 at 1/10 dilution staining DNAJA1 in permeabilized Jurkat cells by Flow cytometry (red). Rabbit IgG negative control (green).
All lanes : Anti-DNAJA1 antibody [EPR7248] (ab126774) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : SKBR 3 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 45 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD