Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11302(B)] to DNAJA2
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Product nameAnti-DNAJA2 antibody [EPR11302(B)]
See all DNAJA2 primary antibodies
DescriptionRabbit monoclonal [EPR11302(B)] to DNAJA2
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
A synthetic peptide corresponding to amino acid residues in Human DNAJA2 was used as an immunogen (UniProt ID O60884).
- HepG2, MCF7, Jurkat and K562 cell lysates. Immunoprecipitation pellet from HepG2 whole cell lysate (ab7900). K562 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab157216 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/50000 - 1/200000. Predicted molecular weight: 46 kDa.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionCo-chaperone of Hsc70.
Sequence similaritiesContains 1 CR-type zinc finger.
Contains 1 J domain.
- Information by UniProt
- Cell cycle progression 3 protein antibody
- Cell cycle progression restoration gene 3 protein antibody
- CPR 3 antibody
Flow cytometric analysis of permeabilized K562 cells labeling DNAJA2 with either ab157216 at 1/10 dilution (red) or a Rabbit IgG negative control (green).
All lanes : Anti-DNAJA2 antibody [EPR11302(B)] (ab157216) at 1/50000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 46 kDa
Anti-DNAJA2 antibody [EPR11302(B)] (ab157216) at 1/50000 dilution + Immunoprecipitation pellet from HepG2 cell lysate at 10 µg
Predicted band size: 46 kDa
ab157216 has been referenced in 1 publication.
- Nillegoda NB et al. Evolution of an intricate J-protein network driving protein disaggregation in eukaryotes. Elife 6:N/A (2017). PubMed: 28504929