Overview

  • Product name
    Anti-DNAJA2 antibody [EPR11302(B)]
    See all DNAJA2 primary antibodies
  • Description
    Rabbit monoclonal [EPR11302(B)] to DNAJA2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to amino acid residues in Human DNAJA2 was used as an immunogen (UniProt ID O60884).

  • Positive control
    • HepG2, MCF7, Jurkat and K562 cell lysates. Immunoprecipitation pellet from HepG2 whole cell lysate (ab7900). K562 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab157216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/200000. Predicted molecular weight: 46 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function
      Co-chaperone of Hsc70.
    • Sequence similarities
      Contains 1 CR-type zinc finger.
      Contains 1 J domain.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cell cycle progression 3 protein antibody
      • Cell cycle progression restoration gene 3 protein antibody
      • CPR 3 antibody
      • CPR3 antibody
      • Dj3 antibody
      • DJA 2 antibody
      • DJA2 antibody
      • DnaJ (Hsp40) homolog subfamily A member 2 antibody
      • DNAJ antibody
      • DnaJ heat shock protein family (Hsp40) member A2 antibody
      • DnaJ homolog subfamily A member 2 antibody
      • DNAJA 2 antibody
      • Dnaja2 antibody
      • DNJ 3 antibody
      • Dnj3 antibody
      • DNJA2_HUMAN antibody
      • HIRA interacting protein 4 antibody
      • HIRA-interacting protein 4 antibody
      • HIRIP 4 antibody
      • HIRIP4 antibody
      • OTTHUMP00000164136 antibody
      • PRO3015 antibody
      • RDJ 2 antibody
      • RDJ2 antibody
      • Renal carcinoma antigen NY REN 14 antibody
      • Renal carcinoma antigen NY-REN-14 antibody
      see all

    Images

    • Flow cytometric analysis of permeabilized K562 cells labeling DNAJA2 with either ab157216 at 1/10 dilution (red) or a Rabbit IgG negative control (green).
    • All lanes : Anti-DNAJA2 antibody [EPR11302(B)] (ab157216) at 1/50000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 46 kDa

    • Anti-DNAJA2 antibody [EPR11302(B)] (ab157216) at 1/50000 dilution + Immunoprecipitation pellet from HepG2 cell lysate at 10 µg

      Predicted band size: 46 kDa

    References

    This product has been referenced in:
    • Nillegoda NB  et al. Evolution of an intricate J-protein network driving protein disaggregation in eukaryotes. Elife 6:N/A (2017). Read more (PubMed: 28504929) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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