Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8756] to DNAJA4
- Suitable for: WB, Flow Cyt, IP
- Reacts with: Mouse, Rat, Human
Product nameAnti-DNAJA4 antibody [EPR8756]
See all DNAJA4 primary antibodies
DescriptionRabbit monoclonal [EPR8756] to DNAJA4
Tested applicationsSuitable for: WB, Flow Cyt, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DNAJA4 aa 350 to the C-terminus. The exact sequence is proprietary.
Database link: Q8WW22
- Human fetal brain and HeLa whole cell lysate (ab150035); HeLa cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab185553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||1/30 - 1/50.|
Sequence similaritiesContains 1 CR-type zinc finger.
Contains 1 J domain.
- Information by UniProt
- DnaJ (Hsp40) homolog subfamily A member 4 antibody
- DnaJ (Hsp40) homolog, subfamily A, member4 antibody
- DNAJ A4 antibody
All lanes : Anti-DNAJA4 antibody [EPR8756] (ab185553) at 1/10000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Hela cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 45 kDa
Blocking buffer: 5% NFDM/TBST
Flow cytometric analysis of DNAJA4 in 2% paraformaldehyde HeLa cells using ab185553 at 1/300 dilution (pink). Secondary antibody: Goat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control: Rabbit monoclonal IgG (green).
Western blot analysis of Immunoprecipitate from Human fetal brain lysate (lane 1) using ab185553 at 1/50 dilution. Lane 2: PBS used instead of Human fetal brain lysates. Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, at 1/1500 dilution. Blocking buffer: 5% NFDM/TBST.
ab185553 has been referenced in 1 publication.
- Sun YZ et al. DNAJA4 deficiency enhances NF-kappa B-related growth arrest induced by hyperthermia in human keratinocytes. J Dermatol Sci N/A:N/A (2018). PubMed: 29807809