Overview

  • Product name

    Anti-DNAJA4 antibody [EPR8756]
    See all DNAJA4 primary antibodies
  • Description

    Rabbit monoclonal [EPR8756] to DNAJA4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DNAJA4 aa 350 to the C-terminus. The exact sequence is proprietary.
    Database link: Q8WW22

  • Positive control

    • Human fetal brain and HeLa whole cell lysate (ab150035); HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab185553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
Flow Cyt 1/300.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/30 - 1/50.

Target

Images

  • All lanes : Anti-DNAJA4 antibody [EPR8756] (ab185553) at 1/10000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Hela cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 45 kDa



    Blocking buffer: 5% NFDM/TBST

  • Flow cytometric analysis of DNAJA4 in 2% paraformaldehyde HeLa cells using ab185553 at 1/300 dilution (pink). Secondary antibody: Goat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control: Rabbit monoclonal IgG (green).

  • Western blot analysis of Immunoprecipitate from Human fetal brain lysate (lane 1) using ab185553 at 1/50 dilution. Lane 2: PBS used instead of Human fetal brain lysates. Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, at 1/1500 dilution.  Blocking buffer: 5% NFDM/TBST.

References

This product has been referenced in:

  • Sun YZ  et al. DNAJA4 deficiency enhances NF-kappa B-related growth arrest induced by hyperthermia in human keratinocytes. J Dermatol Sci N/A:N/A (2018). Read more (PubMed: 29807809) »
See 1 Publication for this product

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