Recombinant Anti-DNAJA4 antibody [EPR8756] (ab185553)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8756] to DNAJA4
- Suitable for: Flow Cyt (Intra), WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-DNAJA4 antibody [EPR8756]
See all DNAJA4 primary antibodies -
Description
Rabbit monoclonal [EPR8756] to DNAJA4 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal brain and HeLa whole cell lysate (ab150035); HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8756 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab185553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/300.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
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IP |
1/30 - 1/50.
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Notes |
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Flow Cyt (Intra)
1/300. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). |
IP
1/30 - 1/50. |
Target
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Sequence similarities
Contains 1 CR-type zinc finger.
Contains 1 J domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 55466 Human
- Entrez Gene: 58233 Mouse
- Entrez Gene: 300721 Rat
- SwissProt: Q8WW22 Human
- SwissProt: Q9JMC3 Mouse
- Unigene: 513053 Human
- Unigene: 489676 Mouse
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Alternative names
- DnaJ (Hsp40) homolog subfamily A member 4 antibody
- DnaJ (Hsp40) homolog, subfamily A, member4 antibody
- DNAJ A4 antibody
see all
Images
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All lanes : Anti-DNAJA4 antibody [EPR8756] (ab185553) at 1/10000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : Hela cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 45 kDaBlocking buffer: 5% NFDM/TBST
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Intracellular flow cytometric analysis of DNAJA4in 2% paraformaldehydeHeLa cellsusing ab185553 at 1/300 dilution (pink). Secondary antibody: Goat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control: Rabbit monoclonal IgG (green).
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Western blot analysis of Immunoprecipitate from Human fetal brain lysate (lane 1) using ab185553 at 1/50 dilution. Lane 2: PBS used instead of Human fetal brain lysates. Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, at 1/1500 dilution. Blocking buffer: 5% NFDM/TBST.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab185553 has been referenced in 1 publication.
- Sun YZ et al. DNAJA4 deficiency enhances NF-kappa B-related growth arrest induced by hyperthermia in human keratinocytes. J Dermatol Sci N/A:N/A (2018). PubMed: 29807809