Key features and details
- Rabbit polyclonal to DNAJB12
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DNAJB12 antibody
DescriptionRabbit polyclonal to DNAJB12
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow
Recombinant fragment corresponding to a region within amino acids 82-353 of Human DNAJB12 (Uniprot ID Q9NXW2)
- A549, H1299, HCT116 and MCF7 cell lysates; Human colon carcinoma tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154410 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 46 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).|
Sequence similaritiesContains 1 J domain.
- Information by UniProt
- DJ10 antibody
- DJB12_HUMAN antibody
- DKFZp586B2023 antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue, labeling DNAJB12 using ab154410 at a 1/500 dilution.
All lanes : Anti-DNAJB12 antibody (ab154410) at 1/1000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : HCT116 whole cell lysate
Lane 4 : MCF7 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 46 kDa
10% SDS PAGE
ab154410 has not yet been referenced specifically in any publications.