Key features and details
- Rabbit polyclonal to DNAJB6
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DNAJB6 antibody
See all DNAJB6 primary antibodies
DescriptionRabbit polyclonal to DNAJB6
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Recombinant protein fragment with a sequence corresponding to a region within amino acids 1 and 126 of Human DNAJB6 (NP_490647).
- HeLaS3 and MOLT4 cell lysates for WB and HeLa cells for IF
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96539 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 36 kDa.|
|ICC/IF||1/100 - 1/200.|
FunctionPlays an indispensable role in the organization of KRT8/KRT18 filaments. Acts as an endogenous molecular chaperone for neuronal proteins including huntingtin. Suppresses aggregation and toxicity of polyglutamine-containing, aggregation-prone proteins. Isoform B but not isoform A inhibits huntingtin aggregation. Has a stimulatory effect on the ATPase activity of HSP70 in a dose-dependent and time-dependent manner and hence acts as a co-chaperone of HSP70. Also reduces cellular toxicity and caspase-3 activity.
Tissue specificityWidely expressed. Highest levels in testis and brain, and lower levels in heart, spleen, intestine, ovary, placenta, lung, kidney, pancreas, thymus, prostate, skeletal muscle, liver and leukocytes. In testis, expressed in germ cells in the earlier stages of differentiation pathway as well as in spermatids. In brain, expressed at a higher level in hippocampus and thalamus and a lower level in amygdala, substantia nigra, corpus callosum and caudate nucleus.
Involvement in diseaseLimb-girdle muscular dystrophy 1E
Sequence similaritiesContains 1 J domain.
DomainThe antiaggregation activity of isoform B resides in the serine-rich region and the C-terminus.
Cellular localizationCytoplasm, perinuclear region. Nucleus. Cytoplasm, myofibril, sarcomere, Z line.
- Information by UniProt
- DJ4 antibody
- DnaJ antibody
- DnaJ (Hsp40) homolog subfamily B member 6 antibody
ab96539 has been referenced in 1 publication.
- Ghaoui R et al. Mutations in HSPB8 causing a new phenotype of distal myopathy and motor neuropathy. Neurology 86:391-8 (2016). PubMed: 26718575