Overview

  • Product name
    Anti-DNAJB6 antibody [EPR17122] - N-terminal
    See all DNAJB6 primary antibodies
  • Description
    Rabbit monoclonal [EPR17122] to DNAJB6 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, WB, Flow Cyt, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human DNAJB6 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: O75190

  • Positive control
    • U87-MG, SH-SY5Y, HeLa and Jurkat lysate. Mouse heart, rat brain, rat heart, C6, RAW264.7 and PC-12 lysate. Human testis tissue. Rat cerebral cortex tissue. SH-SY5Y and Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab198995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/150.
WB 1/1000. Predicted molecular weight: 36 kDa.
Flow Cyt 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/250.
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function
    Plays an indispensable role in the organization of KRT8/KRT18 filaments. Acts as an endogenous molecular chaperone for neuronal proteins including huntingtin. Suppresses aggregation and toxicity of polyglutamine-containing, aggregation-prone proteins. Isoform B but not isoform A inhibits huntingtin aggregation. Has a stimulatory effect on the ATPase activity of HSP70 in a dose-dependent and time-dependent manner and hence acts as a co-chaperone of HSP70. Also reduces cellular toxicity and caspase-3 activity.
  • Tissue specificity
    Widely expressed. Highest levels in testis and brain, and lower levels in heart, spleen, intestine, ovary, placenta, lung, kidney, pancreas, thymus, prostate, skeletal muscle, liver and leukocytes. In testis, expressed in germ cells in the earlier stages of differentiation pathway as well as in spermatids. In brain, expressed at a higher level in hippocampus and thalamus and a lower level in amygdala, substantia nigra, corpus callosum and caudate nucleus.
  • Involvement in disease
    Limb-girdle muscular dystrophy 1E
  • Sequence similarities
    Contains 1 J domain.
  • Domain
    The antiaggregation activity of isoform B resides in the serine-rich region and the C-terminus.
  • Cellular localization
    Cytoplasm, perinuclear region. Nucleus. Cytoplasm, myofibril, sarcomere, Z line.
  • Information by UniProt
  • Database links
  • Alternative names
    • DJ4 antibody
    • DnaJ antibody
    • DnaJ (Hsp40) homolog subfamily B member 6 antibody
    • DNAJ B6 antibody
    • DnaJ homolog subfamily B member 6 antibody
    • DnaJ like 2 protein antibody
    • DNAJB6 antibody
    • DNJB6_HUMAN antibody
    • Heat shock protein J2 antibody
    • HHDJ1 antibody
    • HSJ-2 antibody
    • HSJ2 antibody
    • LGMD1E antibody
    • MRJ antibody
    • MSJ-1 antibody
    • MSJ1 antibody
    see all

Images

  • All lanes : Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995) at 1/1000 dilution

    Lane 1 : U87-MG cell lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 36 kDa

  • Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995) at 1/10000 dilution + Jurkat cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 36 kDa

  • All lanes : Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995) at 1/1000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Rat brain lysate
    Lane 3 : Rat heart lysate
    Lane 4 : C6 cell lysate
    Lane 5 : RAW264.7 cell lysate
    Lane 6 : PC-12 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 36 kDa

  • Immunohistochemical analysis of paraffin-embedded human testis tissue sections labeling DNAJB6 with ab198995 at a 1/250 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue sections labeling DNAJB6 with ab198995 at a 1/250 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.

  • Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y cell line labeling DNAJB6 with ab198995 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized using 0.1% Triton X-100. ab150077 AlexaFluor®488 Goat anti-Rabbit was used as the secondary at a 1/500 dilution. Counterstained using ab7291 anti-Tubulin (mouse mAb) at a 1/1000 dilution with ab150120 AlexaFluor®594 Goat anti-Mouse secondary at a 1/500 dilution.

  • Flow cytometry analysis of DNAJB6 expression in 2% paraformaldehyde-fixed Jurkat (Human acute T cell leukemia) cells cells using ab198995 at 1/500 dilution (red), a Rabbit monoclonal IgG (ab172730) IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody.

  • DNAJB6 was immunoprecipitated from Jurkat (Human acute T cell leukemia) whole cell extract with ab198995 at 1/150 dilution. Western blot was performed from the immunoprecipitate using ab198995 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Jurkat (Human acute T cell leukemia) whole cell extract (Input) 10 µg.
    Lane 2: ab198995 IP in Jurkat (Human acute T cell leukemia) whole cell extract.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab198995 in Jurkat (Human acute T cell leukemia) whole cell extract.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

References

This product has been referenced in:
See all 2 Publications for this product

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