Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15065(B)] to DNAJC11 - C-terminal
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Human
Product nameAnti-DNAJC11 antibody [EPR15065(B)] - C-terminal
See all DNAJC11 primary antibodies
DescriptionRabbit monoclonal [EPR15065(B)] to DNAJC11 - C-terminal
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DNAJC11 aa 500 to the C-terminus. The exact sequence is proprietary.
Database link: Q9NVH1
- HeLa cells and cell lysates; HepG2, SH-SY5Y and A549 cell lysates; Human liver tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab183518 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).|
|IHC-P||1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Sequence similaritiesBelongs to the DNAJC11 family.
Contains 1 J domain.
- Information by UniProt
- dJ126A5.1 antibody
- DJC11_HUMAN antibody
- DnaJ (Hsp40) homolog, subfamily C, member 11 antibody
All lanes : Anti-DNAJC11 antibody [EPR15065(B)] - C-terminal (ab183518) at 1/20000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 63 kDa
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling DNAJC11 with ab183518 at 1/250 dilution. The slide is counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling DNAJC11 with ab183518 at 1/90 dilution. Goat anti-rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control.
ab183518 has not yet been referenced specifically in any publications.