Anti-DNAJC14 antibody (ab121535)
Key features and details
- Rabbit polyclonal to DNAJC14
- Suitable for: ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-DNAJC14 antibody -
Description
Rabbit polyclonal to DNAJC14 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
antigen sequence, corresponding to internal sequence amino acids 396-542 of Human DNAJC14.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab121535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 - 4 µg/ml. | |
| IHC-P | 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Regulates the export of target proteins, such as DRD1, from the endoplasmic reticulum to the cell surface. -
Tissue specificity
Highly expressed in pancreas and selectively expressed in brain, lung, liver, skeletal muscle and kidney. -
Sequence similarities
Contains 1 J domain. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 85406 Human
- Entrez Gene: 74330 Mouse
- Entrez Gene: 114481 Rat
- Omim: 606092 Human
- SwissProt: Q6Y2X3 Human
- SwissProt: Q921R4 Mouse
- SwissProt: Q5XIX0 Rat
- Unigene: 709320 Human
see all -
Alternative names
- DJC14_HUMAN antibody
- DnaJ (Hsp40) homolog, subfamily C, member 14 antibody
- DNAJ antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC14 antibody (ab121535)ab121535 at 1/375 dilution staining DNAJC14 in Paraffin-embedded Human stomach tissue by Immunohistochemistry.
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Immunocytochemistry/ Immunofluorescence - Anti-DNAJC14 antibody (ab121535)Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleoli and nucleus but not nucleoli using ab121535.
Recommended treatment:
Fixation/Permeabilization: PFA/Triton X-100
Protocols
Datasheets and documents
References (1)
ab121535 has been referenced in 1 publication.
- Choi HJ et al. DNAJC14 Ameliorates Inner Ear Degeneration in the DFNB4 Mouse Model. Mol Ther Methods Clin Dev 17:188-197 (2020). PubMed: 31909090
