Overview

  • Product name
    Anti-DNAJC14 antibody
    See all DNAJC14 primary antibodies
  • Description
    Rabbit polyclonal to DNAJC14
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    antigen sequence, corresponding to internal sequence amino acids 396-542 of Human DNAJC14.

  • Positive control
    • Human stomach tissue; RT4, U 251 MG, Human Liver and Tonsil lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab121535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Function
    Regulates the export of target proteins, such as DRD1, from the endoplasmic reticulum to the cell surface.
  • Tissue specificity
    Highly expressed in pancreas and selectively expressed in brain, lung, liver, skeletal muscle and kidney.
  • Sequence similarities
    Contains 1 J domain.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • DJC14_HUMAN antibody
    • DnaJ (Hsp40) homolog, subfamily C, member 14 antibody
    • DNAJ antibody
    • DnaJ homolog subfamily C member 14 antibody
    • DnaJ protein homolog 3 antibody
    • DNAJC14 antibody
    • Dopamine receptor interacting protein antibody
    • Dopamine receptor-interacting protein of 78 kDa antibody
    • DRiP78 antibody
    • hDj 3 antibody
    • HDJ-3 antibody
    • HDJ3 antibody
    • Human DnaJ protein 3 antibody
    • LIP6 antibody
    • LYST interacting protein LIP6 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC14 antibody (ab121535)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC14 antibody (ab121535)
    ab121535 at 1/375 dilution staining DNAJC14 in Paraffin-embedded Human stomach tissue by Immunohistochemistry.
  • Immunocytochemistry/ Immunofluorescence - Anti-DNAJC14 antibody (ab121535)
    Immunocytochemistry/ Immunofluorescence - Anti-DNAJC14 antibody (ab121535)
    Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleoli and nucleus but not nucleoli using ab121535.
    Recommended treatment:
    Fixation/Permeabilization: PFA/Triton X-100
  • Western blot - Anti-DNAJC14 antibody (ab121535)
    Western blot - Anti-DNAJC14 antibody (ab121535)
    All lanes : Anti-DNAJC14 antibody (ab121535) at 1/250 dilution

    Lane 1 : RT 4 cell lysate
    Lane 2 : U 251 MG cell lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human liver tissue lysate
    Lane 5 : Human tonsil tissue lysate

References

ab121535 has not yet been referenced specifically in any publications.

Publishing research using ab121535? Please let us know so that we can cite the reference in this datasheet.

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