Key features and details
- Rabbit polyclonal to DNAJC14
- Suitable for: ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-DNAJC14 antibody
DescriptionRabbit polyclonal to DNAJC14
Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
antigen sequence, corresponding to internal sequence amino acids 396-542 of Human DNAJC14.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionRegulates the export of target proteins, such as DRD1, from the endoplasmic reticulum to the cell surface.
Tissue specificityHighly expressed in pancreas and selectively expressed in brain, lung, liver, skeletal muscle and kidney.
Sequence similaritiesContains 1 J domain.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- DJC14_HUMAN antibody
- DnaJ (Hsp40) homolog, subfamily C, member 14 antibody
- DNAJ antibody
ab121535 at 1/375 dilution staining DNAJC14 in Paraffin-embedded Human stomach tissue by Immunohistochemistry.
Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleoli and nucleus but not nucleoli using ab121535.
Fixation/Permeabilization: PFA/Triton X-100
ab121535 has been referenced in 1 publication.
- Choi HJ et al. DNAJC14 Ameliorates Inner Ear Degeneration in the DFNB4 Mouse Model. Mol Ther Methods Clin Dev 17:188-197 (2020). PubMed: 31909090