Product nameAnti-DNAJC16 antibody
DescriptionRabbit polyclonal to DNAJC16
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
antigen, corresponding to amino acids 633-764 of Human DNAJC16 (UniProt Q9Y2G8).
- Human kidney tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122855 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Sequence similaritiesContains 1 J domain.
Contains 1 thioredoxin domain.
- Information by UniProt
- DJC16_HUMAN antibody
- DnaJ homolog subfamily C member 16 antibody
- Dnajc16 antibody
- KIAA0962 antibody
Immunofluorescent staining of Human cell line U-2 OS shows positivity in vesicles. Recommended concentration of ab122855 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
ab122855, at a 1/50 dilution, staining DNAJC16 in paraffin embedded Human kidney tissue by Immunohistochemistry.
ab122855 has not yet been referenced specifically in any publications.