Overview

  • Product name
  • Description
    Rabbit polyclonal to DNAJC19
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide within Human DNAJC19 aa 62-92 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q96DA6

  • Positive control
    • Mouse kidney tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab175611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 12 kDa.

Target

  • Function
    Probable component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. May act as a co-chaperone that stimulate the ATP-dependent activity.
  • Tissue specificity
    Ubiquitously expressed.
  • Involvement in disease
    Defects in DNAJC19 are the cause of 3-methylglutaconic aciduria type 5 (MGA5) [MIM:610198]; also known as dilated cardiomyopathy with ataxia (DCMA). MGA5 is an autosomal recessive disorder characterized by early-onset dilated cardiomyopathy, growth failure, cerebellar ataxia causing significant motor delays, testicular dysgenesis, growth failure, and significant increases in urine organic acids, particularly 3-methylglutaconic acid and 3-methylglutaric acid.
  • Sequence similarities
    Belongs to the TIM14 family.
    Contains 1 J domain.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DnaJ (Hsp40) homolog, subfamily C, member 19 antibody
    • DnaJ homolog subfamily C member 19 antibody
    • DNAJC19 antibody
    • homolog of yeast TIM14 antibody
    • Mitochondrial import inner membrane translocase subunit TIM14 antibody
    • PAM18 antibody
    • TIM 14 antibody
    • TIM14 antibody
    • TIM14_HUMAN antibody
    • TIMM 14 antibody
    • TIMM14 antibody
    • translocase of the inner mitochondrial membrane 14 antibody
    • Translocase of the inner mitochondrial membrane 14, yeast homolog of antibody
    see all

Images

  • Anti-DNAJC19 antibody (ab175611) at 1/1000 dilution + Mouse kidney tissue lysate at 35 µg

    Predicted band size: 12 kDa

References

ab175611 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (Hela cells)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
30 µg
Treatment
Non treated cells and 25uM AMA for 3h
Specification
Hela cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Arianna Barchiesi

Verified customer

Submitted Jun 17 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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