Key features and details
- Rabbit polyclonal to DNAJC19
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-DNAJC19 antibody
See all DNAJC19 primary antibodies
DescriptionRabbit polyclonal to DNAJC19
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow, Orangutan
- WB: A549, HL-60 and HeLa whole cell lysates; Mouse kidney tissue lysate. IHC-P: Human brain and kidney tissues.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230244 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 12 kDa.|
FunctionProbable component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. May act as a co-chaperone that stimulate the ATP-dependent activity.
Tissue specificityUbiquitously expressed.
Involvement in diseaseDefects in DNAJC19 are the cause of 3-methylglutaconic aciduria type 5 (MGA5) [MIM:610198]; also known as dilated cardiomyopathy with ataxia (DCMA). MGA5 is an autosomal recessive disorder characterized by early-onset dilated cardiomyopathy, growth failure, cerebellar ataxia causing significant motor delays, testicular dysgenesis, growth failure, and significant increases in urine organic acids, particularly 3-methylglutaconic acid and 3-methylglutaric acid.
Sequence similaritiesBelongs to the TIM14 family.
Contains 1 J domain.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- DnaJ (Hsp40) homolog, subfamily C, member 19 antibody
- DnaJ homolog subfamily C member 19 antibody
- DNAJC19 antibody
All lanes : Anti-DNAJC19 antibody (ab230244) at 1/1000 dilution
Lane 1 : Mouse kidney tissue lysate
Lane 2 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 3 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 12 kDa
Observed band size: 12 kDa
Paraffin-embedded human brain tissue stained for DNAJC19 using ab230244 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for DNAJC19 using ab230244 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230244 has not yet been referenced specifically in any publications.