Key features and details
- Rabbit polyclonal to DNAJC21
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DNAJC21 antibody
See all DNAJC21 primary antibodies
DescriptionRabbit polyclonal to DNAJC21
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
Synthetic peptide, corresponding to a region between amino acids 400-450 of Human DNAJC21 (NP_001012339.2)
- Whole cell lysate from HeLa and 293T cells.
General notesAntibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab86434 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/5000. Predicted molecular weight: 62 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMay act as a co-chaperone for HSP70.
Tissue specificityBrain, placenta, kidney and pancreas.
Sequence similaritiesContains 2 C2H2-type zinc fingers.
Contains 1 J domain.
- Information by UniProt
- DJC21_HUMAN antibody
- DnaJ homolog subfamily A member 5 antibody
- DnaJ homolog subfamily C member 21 antibody
All lanes : Anti-DNAJC21 antibody (ab86434) at 0.04 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Predicted band size: 62 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Detection of Human DNAJC21 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86434 at 3 ug/mg lysate. Subsequent Western blot analysis was performed using ab86434 at 1 ug/ml.
ab86434 has been referenced in 1 publication.
- Tummala H et al. DNAJC21 Mutations Link a Cancer-Prone Bone Marrow Failure Syndrome to Corruption in 60S Ribosome Subunit Maturation. Am J Hum Genet 99:115-24 (2016). PubMed: 27346687