• Product name

  • Description

    Rabbit polyclonal to DNAJC2/ZRF1
  • Host species

  • Tested applications

    Suitable for: ICC/IF, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human DNAJC2/ZRF1 aa 500-550.


    Database link: NP_055192.1

  • Positive control

    • HeLa, 293T, Jurkat and Mouse NIH 3T3 whole cell lysates. IF/ICC: LoVo cell line.
  • General notes

     This product was previously labelled as DNAJC2




Our Abpromise guarantee covers the use of ab134572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 0.2 µg/ml.
WB 1/2000 - 1/10000. Predicted molecular weight: 71 kDa.
IP Use at 2-10 µg/mg of lysate.


  • Function

    Acts both as a chaperone in the cytosol and as a chromatin regulator in the nucleus. When cytosolic, acts as a molecular chaperone: component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones HSPA14 that bind to the nascent polypeptide chain. When nuclear, mediates the switching from polycomb-repressed genes to an active state: specifically recruited at histone H2A ubiquitinated at 'Lys-119' (H2AK119ub), and promotes the displacement of the polycomb PRC1 complex from chromatin, thereby facilitating transcription activation. Specifically binds DNA sequence 5'-GTCAAGC-3'.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Contains 1 J domain.
    Contains 2 SANT domains.
  • Domain

    The ZRF1-UBD region specifically recognizes and binds H2AK119ub. The ZRF1-UBD region is also involved in protein-protein interactions with other proteins, suggesting that it may be masked by some regulator, thereby preventing its association with H2AK119ub.
  • Post-translational

    Phosphorylated in M (mitotic) phase.
  • Cellular localization

    Nucleus. Cytoplasm > cytosol.
  • Information by UniProt
  • Database links

  • Alternative names

    • AU020218 antibody
    • DnaJ (Hsp40) homolog subfamily C member 2 antibody
    • DnaJ homolog subfamily C member 2 antibody
    • Dnajc2 antibody
    • DNJC2_HUMAN antibody
    • M phase phosphoprotein 11 antibody
    • M-phase phosphoprotein 11 antibody
    • MGC105894 antibody
    • MIDA1 antibody
    • MPHOSPH11 antibody
    • MPP11 antibody
    • OTTMUSP00000031226 antibody
    • ZRF1 antibody
    • Zrf2 antibody
    • ZUO1 antibody
    • Zuotin related factor 1 antibody
    • Zuotin related factor 2 antibody
    • Zuotin-related factor 1 antibody
    see all


  • All lanes : Anti-DNAJC2/ZRF1 antibody (ab134572) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg
    Lane 5 : NIH 3T3 whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 71 kDa

    Exposure time: 10 seconds
  • ab134572 stained LoVo cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab134572 at 0.2µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Detection of DNAJC2/ZRF1 by Western Blot of Immunprecipitate.
    Immunoprecipitation of HeLa whole cell lysate using ab134572 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) followed by detection of DNAJC2/ZRF1 with ab134572 at 1µg/ml. The lysate in lane 2 is precipitated with an IgG control antibody. Detection: Chemiluminescence with exposure time of 3 seconds.


ab134572 has not yet been referenced specifically in any publications.

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