Key features and details
- Rabbit polyclonal to DNAJC2/ZRF1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-DNAJC2/ZRF1 antibody
See all DNAJC2/ZRF1 primary antibodies
DescriptionRabbit polyclonal to DNAJC2/ZRF1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
- HeLa, 293T, Jurkat and Mouse NIH 3T3 whole cell lysates. IF/ICC: LoVo cell line.
This product was previously labelled as DNAJC2
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8.
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab134572 was affinity purified using an epitope specific to DNAJC2/ZRF1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab134572 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 71 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionActs both as a chaperone in the cytosol and as a chromatin regulator in the nucleus. When cytosolic, acts as a molecular chaperone: component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones HSPA14 that bind to the nascent polypeptide chain. When nuclear, mediates the switching from polycomb-repressed genes to an active state: specifically recruited at histone H2A ubiquitinated at 'Lys-119' (H2AK119ub), and promotes the displacement of the polycomb PRC1 complex from chromatin, thereby facilitating transcription activation. Specifically binds DNA sequence 5'-GTCAAGC-3'.
Tissue specificityWidely expressed.
Sequence similaritiesContains 1 J domain.
Contains 2 SANT domains.
DomainThe ZRF1-UBD region specifically recognizes and binds H2AK119ub. The ZRF1-UBD region is also involved in protein-protein interactions with other proteins, suggesting that it may be masked by some regulator, thereby preventing its association with H2AK119ub.
modificationsPhosphorylated in M (mitotic) phase.
Cellular localizationNucleus. Cytoplasm > cytosol.
- Information by UniProt
- AU020218 antibody
- DnaJ (Hsp40) homolog subfamily C member 2 antibody
- DnaJ homolog subfamily C member 2 antibody
All lanes : Anti-DNAJC2/ZRF1 antibody (ab134572) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 71 kDa
Exposure time: 10 seconds
Detection of DNAJC2/ZRF1 by Western Blot of Immunprecipitate.
Immunoprecipitation of HeLa whole cell lysate using ab134572 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) followed by detection of DNAJC2/ZRF1 with ab134572 at 1µg/ml. The lysate in lane 2 is precipitated with an IgG control antibody. Detection: Chemiluminescence with exposure time of 3 seconds.
ab134572 has not yet been referenced specifically in any publications.