Anti-DNAJC3 antibody (ab154714)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-DNAJC3 antibody
See all DNAJC3 primary antibodies -
Description
Rabbit polyclonal to DNAJC3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 270-492 of Human DNAJC3 (UniProt Q13217).
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Positive control
- 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab154714 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 58 kDa. |
Target
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Function
Involved in the unfolded protein response (UPR) during ER stress. Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity. -
Tissue specificity
Widely expressed with high level in the pancreas and testis. Also expressed in cell lines with different levels. -
Sequence similarities
Contains 1 J domain.
Contains 9 TPR repeats. -
Domain
The J domain mediates interaction with HSPA8. -
Cellular localization
Endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 286770 Cow
- Entrez Gene: 5611 Human
- Entrez Gene: 100037258 Mouse
- Entrez Gene: 100154166 Pig
- Entrez Gene: 63880 Rat
- Omim: 601184 Human
- SwissProt: Q27968 Cow
- SwissProt: Q13217 Human
see all -
Alternative names
- DnaJ (Hsp40) homolog subfamily C member 3 antibody
- DnaJ homolog subfamily C member 3 antibody
- DNAJC3 antibody
see all
Images
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Western blot - Anti-DNAJC3 antibody (ab154714)All lanes : Anti-DNAJC3 antibody (ab154714) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : MOLT4 whole cell lysate
Lane 5 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 58 kDa
7.5% SDS PAGE
Protocols
References
ab154714 has not yet been referenced specifically in any publications.
