Name: Anti-DNAJC3 antibody
SKU: ab154714

Product name

Anti-DNAJC3 antibody
See all DNAJC3 primary antibodies
Description

Rabbit polyclonal to DNAJC3
Host species

Rabbit
Tested applications

Suitable for: WBmore details
Species reactivity

Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig
Immunogen

Recombinant fragment, corresponding to a region within amino acids 270-492 of Human DNAJC3 (UniProt Q13217).
Positive control
- 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
Concentration information loading...
Purity

Immunogen affinity purified
Clonality

Polyclonal
Isotype

IgG
Research areas

Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
Positive Controls

Our Abpromise guarantee covers the use of ab154714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/500 - 1/3000. Predicted molecular weight: 58 kDa.

Function

Involved in the unfolded protein response (UPR) during ER stress. Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity.
Tissue specificity

Widely expressed with high level in the pancreas and testis. Also expressed in cell lines with different levels.
Sequence similarities

Contains 1 J domain.
Contains 9 TPR repeats.
Domain

The J domain mediates interaction with HSPA8.
Cellular localization

Endoplasmic reticulum.
Target information above from: UniProt accession Q13217 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 286770 Cow
- Entrez Gene: 5611 Human
- Entrez Gene: 100037258 Mouse
- Entrez Gene: 100154166 Pig
- Entrez Gene: 63880 Rat
- Omim: 601184 Human
- SwissProt: Q27968 Cow
- SwissProt: Q13217 Human
- SwissProt: Q91YW3 Mouse
- SwissProt: Q9R0T3 Rat
- Unigene: 59214 Human
- Unigene: 12616 Mouse
- Unigene: 162234 Rat
see all
Alternative names
- DnaJ (Hsp40) homolog subfamily C member 3 antibody
- DnaJ homolog subfamily C member 3 antibody
- DNAJC3 antibody
- DNJC3_HUMAN antibody
- double-stranded RNA-activated protein kinase inhibitor antibody
- Endoplasmic reticulum DnaJ protein 6 antibody
- ERdj6 antibody
- HP58 antibody
- Interferon induced, double stranded RNA activated protein kinase inhibitor antibody
- Interferon-induced antibody
- P58 antibody
- p58 ipk antibody
- P58IPK antibody
- PRKRI antibody
- Protein kinase inhibitor of 58 kDa antibody
- Protein kinase inhibitor p58 antibody
- Protein kinase interferon inducible double stranded RNA dependent inhibitor antibody
see all

Western blot - Anti-DNAJC3 antibody (ab154714)

All lanes : Anti-DNAJC3 antibody (ab154714) at 1/1000 dilution

Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : MOLT4 whole cell lysate
Lane 5 : Raji whole cell lysate

Lysates/proteins at 30 µg per lane.

Predicted band size: 58 kDa

7.5% SDS PAGE

Western blot protocols

ab154714 has not yet been referenced specifically in any publications.

Publishing research using ab154714? Please let us know so that we can cite the reference in this datasheet.

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Anti-DNAJC3 antibody (ab154714)

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