Key features and details
- Rabbit polyclonal to DNAJC3
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
Product nameAnti-DNAJC3 antibody
See all DNAJC3 primary antibodies
DescriptionRabbit polyclonal to DNAJC3
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Rhesus monkey
Recombinant fragment within Human DNAJC3 (internal sequence). The exact sequence is proprietary.
Database link: Q13217
- WB: Raji whole cell lysate. IHC-P: Human colon carcinoma tissue. ICC/IF: A431 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227140 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 58 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionInvolved in the unfolded protein response (UPR) during ER stress. Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity.
Tissue specificityWidely expressed with high level in the pancreas and testis. Also expressed in cell lines with different levels.
Sequence similaritiesContains 1 J domain.
Contains 9 TPR repeats.
DomainThe J domain mediates interaction with HSPA8.
Cellular localizationEndoplasmic reticulum.
- Information by UniProt
- DnaJ (Hsp40) homolog subfamily C member 3 antibody
- DnaJ homolog subfamily C member 3 antibody
- DNAJC3 antibody
Anti-DNAJC3 antibody (ab227140) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg
Predicted band size: 58 kDa
7.5% SDS-PAGE gel.
Paraffin-embedded human colon carcinoma tissue stained for DNAJC3 using ab227140 at 1/250 dilution in immunohistochemical analysis.
Ice-cold methanol-fixed A431 (human epidermoid carcinoma cell line) cells stained for DNAJC3 (green) using ab227140 at 1/500 dilution in ICC/IF. Nuclear Counterstain: Hoechst 33343.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227140 has not yet been referenced specifically in any publications.