Overview

  • Product name

  • Description

    Rabbit polyclonal to DNAJC3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human DNAJC3 (internal sequence). The exact sequence is proprietary.
    Database link: Q13217

  • Positive control

    • WB: Raji whole cell lysate. IHC-P: Human colon carcinoma tissue. ICC/IF: A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227140 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 58 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Involved in the unfolded protein response (UPR) during ER stress. Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity.
  • Tissue specificity

    Widely expressed with high level in the pancreas and testis. Also expressed in cell lines with different levels.
  • Sequence similarities

    Contains 1 J domain.
    Contains 9 TPR repeats.
  • Domain

    The J domain mediates interaction with HSPA8.
  • Cellular localization

    Endoplasmic reticulum.
  • Information by UniProt
  • Database links

  • Alternative names

    • DnaJ (Hsp40) homolog subfamily C member 3 antibody
    • DnaJ homolog subfamily C member 3 antibody
    • DNAJC3 antibody
    • DNJC3_HUMAN antibody
    • double-stranded RNA-activated protein kinase inhibitor antibody
    • Endoplasmic reticulum DnaJ protein 6 antibody
    • ERdj6 antibody
    • HP58 antibody
    • Interferon induced, double stranded RNA activated protein kinase inhibitor antibody
    • Interferon-induced antibody
    • P58 antibody
    • p58 ipk antibody
    • P58IPK antibody
    • PRKRI antibody
    • Protein kinase inhibitor of 58 kDa antibody
    • Protein kinase inhibitor p58 antibody
    • Protein kinase interferon inducible double stranded RNA dependent inhibitor antibody
    see all

Images

  • Anti-DNAJC3 antibody (ab227140) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg

    Predicted band size: 58 kDa



    7.5% SDS-PAGE gel.

  • Paraffin-embedded human colon carcinoma tissue stained for DNAJC3 using ab227140 at 1/250 dilution in immunohistochemical analysis.

  • Ice-cold methanol-fixed A431 (human epidermoid carcinoma cell line) cells stained for DNAJC3 (green) using ab227140 at 1/500 dilution in ICC/IF. Nuclear Counterstain: Hoechst 33343.

References

ab227140 has not yet been referenced specifically in any publications.

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