Product nameAnti-DNAJC3 antibody [EP597Y]
See all DNAJC3 primary antibodies
DescriptionRabbit monoclonal [EP597Y] to DNAJC3
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Human
Synthetic peptide within Human DNAJC3 aa 1-100 (N terminal). The exact sequence is proprietary.
- HeLa cell lysate.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab52899 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 58 kDa).|
FunctionInvolved in the unfolded protein response (UPR) during ER stress. Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity.
Tissue specificityWidely expressed with high level in the pancreas and testis. Also expressed in cell lines with different levels.
Sequence similaritiesContains 1 J domain.
Contains 9 TPR repeats.
DomainThe J domain mediates interaction with HSPA8.
Cellular localizationEndoplasmic reticulum.
- Information by UniProt
- DnaJ (Hsp40) homolog subfamily C member 3 antibody
- DnaJ homolog subfamily C member 3 antibody
- DNAJC3 antibody
Anti-DNAJC3 antibody [EP597Y] (ab52899) at 1/1000 dilution + HeLa cell lysate at 10 µg
goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 58 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
ab52899 has not yet been referenced specifically in any publications.