Overview

  • Product name

  • Description

    Rabbit polyclonal to DNAJC6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 260-290 of Human DNAJC6.

  • Positive control

    • K562 cell line lysates. Mouse brain tissue lysates. Human brain tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody is purified through a protein A column, followed by peptide affinity purification.
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab103321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 100 kDa.

Target

  • Relevance

    DNAJC6 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus, a glycine/phenylalanine (G/F)-rich region, and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain.
  • Database links

  • Alternative names

    • auxilin antibody
    • DJC6 antibody
    • DnaJ (Hsp40) homolog, subfamily B, member 6 antibody
    • DnaJ (Hsp40) homolog, subfamily C, member 6 antibody
    • DnaJ homolog subfamily C member 6 antibody
    • EC 3.1.3.48 antibody
    • KIAA0473 antibody
    • MGC129914 antibody
    • MGC129915 antibody
    • MGC48436 antibody
    • putative tyrosine-protein phosphatase auxilin antibody
    see all

Images

  • Anti-DNAJC6 antibody (ab103321) at 1/100 dilution + K562 cell line lysates at 35 µg

    Predicted band size: 100 kDa

  • Anti-DNAJC6 antibody (ab103321) at 1/100 dilution + Mouse brain tissue lysates at 35 µg

    Predicted band size: 100 kDa

  • ab103321 at 1/50 dilution staining DNAJC6 by immunohistochemistry in formalin-fixed and paraffin-embedded Human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

This product has been referenced in:

  • Maib H  et al. Cargo regulates clathrin-coated pit invagination via clathrin light chain phosphorylation. J Cell Biol 217:4253-4266 (2018). Read more (PubMed: 30228161) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunoprecipitation
Sample
Mouse Tissue lysate - nuclear (Mouse (C57BL6) cortex homogenate)
Total protein in input
100 µg
Treatment
with 10mgThiamEt G (OGA inhibitor)/kg for 3hrs after IP injection
Immuno-precipitation step
Protein G
Specification
Mouse (C57BL6) cortex homogenate

Abcam user community

Verified customer

Submitted Sep 13 2019

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