Product nameAnti-DNAJC6 antibody
See all DNAJC6 primary antibodies
DescriptionRabbit polyclonal to DNAJC6
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
- WB: Rt-4 and U-251 MG cell lysates; ICC/IF: U-2OS cells; IHC-P: Human cerebral cortex tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab262876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
|WB||Use a concentration of 0.4 µg/ml.|
RelevanceDNAJC6 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus, a glycine/phenylalanine (G/F)-rich region, and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain.
- auxilin antibody
- DJC6 antibody
- DnaJ (Hsp40) homolog, subfamily B, member 6 antibody
Lane 1 : Marker
Lanes 2-3 : Anti-DNAJC6 antibody (ab262876) at 0.4 µg/ml
Lane 2 : RT-4 cell lysate
Lane 3 : U-251 MG sp cell lysate
PFA-fixed, Triton X-100 permeabilized human U-2OS cells stained for DNAJC6 (green) using ab262876 at 4 µg/ml in ICC/IF.
Paraffin-embedded Human cerebral cortex tissue stained for DNAJC6 using ab262876 at 1/2500 dilution in immunohistochemical analysis.
Paraffin-embedded Human pancreas tissue stained for DNAJC6 using ab262876 at 1/2500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab262876 has not yet been referenced specifically in any publications.