Recombinant
RabMAb

Recombinant Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830)

Overview

  • Product name

    Anti-DNAJC7 antibody [EPR13349] - N-terminal
    See all DNAJC7 primary antibodies
  • Description

    Rabbit monoclonal [EPR13349] to DNAJC7 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human DNAJC7 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q99615

  • Positive control

    • HeLa, HepG2, 293T and Jurkat cell lysates; Human breast tissue and colonic adenocarcinoma tissue; HepG2 and HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR13349
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab179830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/10 - 1/100.

Target

  • Function

    Acts as co-chaperone regulating the molecular chaperones HSP70 and HSP90 in folding of steroid receptors, such as the glucocorticoid receptor and the progesterone receptor. Proposed to act as a recycling chaperone by facilitating the return of chaperone substrates to early stages of chaperoning if further folding is required. In vitro, induces ATP-independent dissociation of HSP90 but not of HSP70 from the chaperone-substrate complexes. Recruits NR1I3 to the cytoplasm.
  • Sequence similarities

    Contains 1 J domain.
    Contains 9 TPR repeats.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasm > cytoskeleton. Colocalizes with NR1I3 to microtubules.
  • Information by UniProt
  • Database links

  • Alternative names

    • DJ11 antibody
    • DJC7 antibody
    • DnaJ (Hsp40) homolog subfamily C member 7 antibody
    • DnaJ homolog subfamily C member 7 antibody
    • Dnajc7 antibody
    • DNJC7_HUMAN antibody
    • Tetratricopeptide repeat domain 2 antibody
    • Tetratricopeptide repeat protein 2 antibody
    • TPR repeat protein 2 antibody
    • TPR2 antibody
    • TTC2 antibody
    see all

Images

  • All lanes : Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 56 kDa

  • Immunofluorescent analysis of HepG2 cells labeling DNAJC7 with ab179830 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling DNAJC7 with ab179830 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot analysis on immunoprecipitation pellet from (1) 293T cell lysate or (2) 1X PBS (negative control) immunoprecipitated using ab179830 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Flow cytometric analysis of permeabilized HeLa cells labeling DNAJC7 with ab179830 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

  • Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling DNAJC7 with ab179830 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

References

ab179830 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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