Anti-DnaK antibody [8E2/2] (ab69617)
Key features and details
- Mouse monoclonal [8E2/2] to DnaK
- Suitable for: WB
- Reacts with: Escherichia coli
- Isotype: IgG1
Overview
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Product name
Anti-DnaK antibody [8E2/2]
See all DnaK primary antibodies -
Description
Mouse monoclonal [8E2/2] to DnaK -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Escherichia coli -
Immunogen
Full length protein corresponding to Escherichia coli DnaK.
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General notes
This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
8E2/2 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab69617 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa). |
Target
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Relevance
DnaK is the prokaryotic analogue of eukaryotic Hsp70. Heat shock proteins applies to a group of proteins that assist in the assembly, folding, and translocation of other proteins. In addition, they protect the cell against heat injury or other forms of stress. All cells, prokaryotic and eukaryotic, are able to respond to different cellular stresses by synthesizing these proteins. Heat shock proteins are highly conserved, ubiquitously distributed, and involved in important aspects of viral and bacterial infections, autoimmune diseases, and in cancer immunity. Two families of molecular chaperones have been identified. The members of the Hsp70 family (DnaK/DnaJ/GrpE) bind to the growing polypeptide chain and prevent its premature folding. The chaperonin family (GroEL and GroES) assists in correct folding when the complete polypeptide chain is formed and is transported into the cytosol or mitochondria. All the major heat shock proteins help to suppress irreversible unfolding reactions. These protein folding 'assistants' may have important functions in amyloid diseases where incorrectly folded proteins accumulate as folded aggregates. -
Cellular localization
Cytoplasm. Cell inner membrane; Peripheral membrane protein. -
Database links
- Entrez Gene: 944750 Escherichia coli
- SwissProt: P0A6Y8 Escherichia coli
- SwissProt: Q1RGI8 Escherichia coli
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Alternative names
- Chaperone Hsp70 antibody
- Chaperone protein dnaK antibody
- Co chaperone with DnaJ antibody
see all
Images
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All lanes :
Lane 1 : DnaK active recombinant protein
Lane 2 : E.coli cell lysate.
Predicted band size: 70 kDaThis image was generated using the ascites version of the product.
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Anti-DnaK antibody [8E2/2] (ab69617) at 0.1 µg/ml + E.Coli DnaK protein
Predicted band size: 70 kDa
Observed band size: 70 kDaThis image was generated using the ascites version of the product.
Datasheets and documents
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SDS download
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Datasheet download
References (34)
ab69617 has been referenced in 34 publications.
- Liu J et al. Mechanistic insights into the subversion of the linear ubiquitin chain assembly complex by the E3 ligase IpaH1.4 of Shigella flexneri. Proc Natl Acad Sci U S A 119:e2116776119 (2022). PubMed: 35294289
- Cohen H et al. Intracellular Salmonella Paratyphi A is motile and differs in the expression of flagella-chemotaxis, SPI-1 and carbon utilization pathways in comparison to intracellular S. Typhimurium. PLoS Pathog 18:e1010425 (2022). PubMed: 35381053
- Feng L et al. Elucidation of a complete mechanical signaling and virulence activation pathway in enterohemorrhagic Escherichia coli. Cell Rep 39:110614 (2022). PubMed: 35385749
- Kim JS et al. Promiscuity of response regulators for thioredoxin steers bacterial virulence. Nat Commun 13:6210 (2022). PubMed: 36266276
- Maeda DLNF et al. Killed whole-genome reduced-bacteria surface-expressed coronavirus fusion peptide vaccines protect against disease in a porcine model. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33858942