• Product name
    Anti-DnaK antibody [8E2/2]
    See all DnaK primary antibodies
  • Description
    Mouse monoclonal [8E2/2] to DnaK
  • Host species
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Full length protein (Escherichia coli )


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab69617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).


  • Relevance
    DnaK is the prokaryotic analogue of eukaryotic Hsp70. Heat shock proteins applies to a group of proteins that assist in the assembly, folding, and translocation of other proteins. In addition, they protect the cell against heat injury or other forms of stress. All cells, prokaryotic and eukaryotic, are able to respond to different cellular stresses by synthesizing these proteins. Heat shock proteins are highly conserved, ubiquitously distributed, and involved in important aspects of viral and bacterial infections, autoimmune diseases, and in cancer immunity. Two families of molecular chaperones have been identified. The members of the Hsp70 family (DnaK/DnaJ/GrpE) bind to the growing polypeptide chain and prevent its premature folding. The chaperonin family (GroEL and GroES) assists in correct folding when the complete polypeptide chain is formed and is transported into the cytosol or mitochondria. All the major heat shock proteins help to suppress irreversible unfolding reactions. These protein folding 'assistants' may have important functions in amyloid diseases where incorrectly folded proteins accumulate as folded aggregates.
  • Cellular localization
    Cytoplasm. Cell inner membrane; Peripheral membrane protein.
  • Database links
    • Alternative names
      • Chaperone Hsp70 antibody
      • Chaperone protein dnaK antibody
      • Co chaperone with DnaJ antibody
      • dnaK antibody
      • Heat shock 70 kDa protein antibody
      • HSP70 antibody
      see all


    • All lanes :

      Lane 1 : DnaK active recombinant protein
      Lane 2 : E.coli cell lysate.

      Predicted band size: 70 kDa

    • Anti-DnaK antibody [8E2/2] (ab69617) at 0.1 µg/ml + E.Coli DnaK protein

      Predicted band size: 70 kDa
      Observed band size: 70 kDa


    This product has been referenced in:
    • Yang B  et al. Transcriptional Activator GmrA, Encoded in Genomic Island OI-29, Controls the Motility of EnterohemorrhagicEscherichia coliO157:H7. Front Microbiol 9:338 (2018). Read more (PubMed: 29520261) »
    • Aviv G  et al. The plasmid-encoded Ipf and Klf fimbriae display different expression and varying roles in the virulence of Salmonella enterica serovar Infantis in mouse vs. avian hosts. PLoS Pathog 13:e1006559 (2017). Read more (PubMed: 28817673) »

    See all 14 Publications for this product

    Customer reviews and Q&As

    Western blot
    Sodalis glossinidius Cell lysate - whole cell (bacteria)
    Loading amount
    30 µg
    42C heatshock for 5 min
    Gel Running Conditions
    Reduced Denaturing (12.5% SDS-PAGE)
    Blocking step
    Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

    Laura Runyen-Janecky

    Verified customer

    Submitted Mar 01 2013

    Expiration date: March 27, 2013
    Value: $337

    We do not have data for that species but I am very pleased to hear you would like to accept our offer and test ab69617 on samples of Sodalis glossinidius. This code wi...

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    Thank you for your reply.

    You are correct that you have to purchase the antibody in question first (at full price, but after you have submitted the Abreview on the antibody performed (and it can be positive or negative), you can use the code ...

    Read More

    Thank you for contacting Abcam
    To our knowledge, ab69617 has not been tested in with other bacterial species, including the Sodlalis DnaK protein. However by participating in our AbTrial program you can now use our products in an untested a...

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    DISCOUNT CODE: ******** Expiration date: DD MM YYYY I am very pleased to hear you would like to accept our offer and test ab69617 in Xanthomonas campestris pv. Campestris. The homology between e. coli DnaK and Xcc DnaK is 72.6% (see attached pdf fo...

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    Thank you and your customer for providing the sequence of DnaK from Xanthomonas campestris pv. Campestris. Both antibodies were generated with the full-length DnaK protein; ab69617 with E.coli DnaK and ab73473 with DnaK from Mycobacterium avium ssp....

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    Thank you for your inquiry. Unfortunately, I do not have access to the sequence of DnaK of Xanthomonas campestris pv. Campestris. Could you please ask your customer to provide me with this sequence? I will then make an alignment. If the homolo...

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