Anti-DnaK antibody (ab80161)
Key features and details
- Rabbit polyclonal to DnaK
- Suitable for: WB
- Reacts with: Chlamydomonas reinhardtii
- Isotype: IgG
Overview
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Product name
Anti-DnaK antibody
See all DnaK primary antibodies -
Description
Rabbit polyclonal to DnaK -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Chlamydomonas reinhardtii -
Immunogen
Recombinant full length DnaK of Chlamydomonas reinhardtii
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Positive control
- WB: Arabidopsis thaliana leaf extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab80161 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000. Detects a band of approximately 70 kDa.
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Notes |
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WB
1/5000. Detects a band of approximately 70 kDa. |
Target
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Relevance
DnaK is the prokaryotic analogue of eukaryotic Hsp70. Heat shock proteins applies to a group of proteins that assist in the assembly, folding, and translocation of other proteins. In addition, they protect the cell against heat injury or other forms of stress. All cells, prokaryotic and eukaryotic, are able to respond to different cellular stresses by synthesizing these proteins. Heat shock proteins are highly conserved, ubiquitously distributed, and involved in important aspects of viral and bacterial infections, autoimmune diseases, and in cancer immunity. Two families of molecular chaperones have been identified. The members of the Hsp70 family (DnaK/DnaJ/GrpE) bind to the growing polypeptide chain and prevent its premature folding. The chaperonin family (GroEL and GroES) assists in correct folding when the complete polypeptide chain is formed and is transported into the cytosol or mitochondria. All the major heat shock proteins help to suppress irreversible unfolding reactions. These protein folding 'assistants' may have important functions in amyloid diseases where incorrectly folded proteins accumulate as folded aggregates. -
Cellular localization
Cytoplasm. Cell inner membrane; Peripheral membrane protein. -
Alternative names
- Chaperone Hsp70 antibody
- Chaperone protein dnaK antibody
- Co chaperone with DnaJ antibody
see all
Images
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Lane 1. 15 µg of Arabidopsis thaliana leaf extract.
Lane 2. 10 µg of total protein from: Synechocystis 6803 motile.
Lane 3. Synechocystis 6803 GT (glucose tolerant strain).
Lane 4. Synechococcus elongates 7942.
Marker – Pierce™ Pre-stained Protein MW Marker (kat #26612)
Total protein was extracted with following buffer: 10 mM Tris HCl, pH 8.0, 0.5% LDS, 4% glycerol, 0.1 mM EDTA were mixed with sample buffer and denatured for 5 min at 95°C. Samples were separated on 10% SDS -PAGE and blotted for 1h to nitrocellulose membrane (Amersham Protran) using tank wet transfer (Bio-Rad) in standard transfer buffer in the presence of 20% methanol. Transfer of proteins to the membrane was checked using 0.5% Ponceau S staining before the blocking step. Blots were blocked in buffer (2% low-fat milk in 1xPBS, 0.1% Tween) for 1 h at room temperature (RT) with agitation. Blots were incubated with the primary antibody (ab80161) at a dilution of 1/5000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in PBS-T at RT with agitation. The blot was incubated in secondary antibody goat anti-rabbit IgG, diluted to 1/30000 for 1h at RT with agitation. The blot was washed as above and developed for 5 min with Clarity Western ECL Substrate and ChemiDoc detection system (Bio-Rad).
Datasheets and documents
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Datasheet download
References (3)
ab80161 has been referenced in 3 publications.
- Zhi Y et al. ptsI gene in the phosphotransfer system is a potential target for developing a live attenuated Salmonella vaccine. Int J Mol Med 45:1327-1340 (2020). PubMed: 32323733
- Ghosh S et al. Proteometabolomic Study of Compatible Interaction in Tomato Fruit Challenged with Sclerotinia rolfsii Illustrates Novel Protein Network during Disease Progression. Front Plant Sci 7:1034 (2016). WB . PubMed: 27507973
- Thompson AD et al. Visualization and functional analysis of the oligomeric states of Escherichia coli heat shock protein 70 (Hsp70/DnaK). Cell Stress Chaperones 17:313-27 (2012). PubMed: 22076723